Louis Gardner Email & Phone Number

Rockville, Maryland, United States

Preclinical Development and Bioanalytical Sciences

Rockville, Maryland

GLP Assay Development

Clinical and Bio-defense assay development

Performed initial developmental work for an RT-PCR C. diff clinical assay.Core member of a small R&D team that developed and launched the Argus Optical Mapping System;, concentrating on the following areas:- Image acquisition via a fluorescence microscopy-based system (Optical Mapper).- Linear DNA deposition and subsequent restriction endonuclease digestion on a charged glass substrate.- Increased the stability of key consumables from 2 weeks to 6 months.- Validated system… Show more Performed initial developmental work for an RT-PCR C. diff clinical assay.Core member of a small R&D team that developed and launched the Argus Optical Mapping System;, concentrating on the following areas:- Image acquisition via a fluorescence microscopy-based system (Optical Mapper).- Linear DNA deposition and subsequent restriction endonuclease digestion on a charged glass substrate.- Increased the stability of key consumables from 2 weeks to 6 months.- Validated system for commercial launch.Provided on-site and experimental troubleshooting support for customers post-launch.Summarized experimental results and presented data to the research and management team.Developed and transferred the QC protocol and specs to the Manufacturing department for the Argus (fluorescence microscopy and image processing instrument). Show less

Essential to the formation of OpGen's high-throughput mapping services lab.Trained and supervised a team of 4-6 Lab Associates.Facilitated the validation and transfer of tech from R&D to production.

Evaluated the procedures used for Optical Mapping to identify areas where efficiency, scale, cost and reliability could be improved.Led scale-up initiative for OpGen's optical surface manufacturing, increasing yield 5-fold while maintaining performance specifications.

Extracted high molecular weight DNA from bacterial cells.Performed restriction endonuclease digestion of single-molecule DNA bound to a charged glass substrate.Assembled data from digested DNA to create whole-genome restriction maps.

Created, maintained and characterized congenic rat strains for use in recombinant screens and carcinogen resistance phenotyping studies (breeding colonies of over 1,500 animals).Trained and supervised undergraduate students in animal colony management and molecular biology skills.- DNA/RNA extraction from animal tissue.- Genotyped congenic rat lines through PCR and gel electrophoresis.Performed necropsies and collected tissues from rats.Assisted in sorting of cells using… Show more Created, maintained and characterized congenic rat strains for use in recombinant screens and carcinogen resistance phenotyping studies (breeding colonies of over 1,500 animals).Trained and supervised undergraduate students in animal colony management and molecular biology skills.- DNA/RNA extraction from animal tissue.- Genotyped congenic rat lines through PCR and gel electrophoresis.Performed necropsies and collected tissues from rats.Assisted in sorting of cells using flow cytometry and magnetic columns for RNA expression analysis.Managed ENU mutagenesis and yeast-based screening assay to produce knockout rats. Show less