Qualification - Master of Research (M.Res.) in NeurotechnologyPI: Prof. Rylie GreenProject - Closed-Loop Brain-Computer Interface (BCIs) for Modulation of Cognitive Functions

Neuroscience and Life Science content creationEngagements:Primary Production Site (Instagram)- Educational contents and information- Non-associated product review- Regional life science news analysis- Frontier findings interpretationProgress:Website establishment, Reviewed Materials Data Base, Product Retail

Hand Health and Fitness Product DesignEngagementsPrevious Stage - Market Analysis, Product Research, Consultation, Productr Design, 3D modelling, Prototype ManufacturingCurrent Stage - Patent Approval Coming Stage - Mass Production, Marketing

Qualification - Master of Philosophy (M.Phil.)PI: Prof. Julie Semmelhack (Ph.D. - UCSD)Research Project - Neural circuits for defensive action selection in zebrafishEngagements:(Research)Cross Breeding, Maintenance, and Preparation of Model Organism in Zebrafish- Cross-selective targets of Zebrafish to yield transparent larvae for two-photon imaging- Selections of crossing between Wildtype, genetically modified (GCaMP), and Nacre+/- & Nacre-/- Models.- Replacement, monitoring, and control of solution for the new generation of embryos and larvae- Embedment of larvae via agarose on coverslips for imaging in behavioral and two-photon experimentsObservation and Assessment of Visual Processing and Behavioral Response in Larvae via Optogenetics- Record behavioral responses corresponding to different stimulation and hypothesis- Engage and run Python programming with inputs in modifying selective parameters(Technical)- Design experimental setup of model holding chamber using CAD software (Solidworks, FreeCAD)- Hardware testing and set-up actualization using magnetic slide-snap mechanism- Prototype of hardware and improvement of set up along generations***Program withdrawn and transferred

Final Year Project Student Researcher (Neuroregenerative Medicine Team)Research Project:Neural Differentiation of Adipose-derived Mesenchymal Stem Cells by NanomatricesSupervised by Prof. YUNG, K.L. Ken (Oxford, PhD - Neuroscience) & Dr. TAM, S.W. Cathy (HKU, PhD - Neuroscience)Engagements:- Experiment with patented nano-matrcies- Investigate the optimal configuration and composition of nano-matrices for neural differentiation- Formulate the optimal culture media composition for neural lineage differentiation enhancement- Manipulate Adipose-derived Mesenchymal Stem Cells (ADMSCs)to differentiate into the neural lineage- Examination by Immunocytochemistry and Fluorescent Imaging- Quantitative data analysis by using software Prism and ImageJResults:Successful differentiate of ADMSCs into neural progenitor cells with nano-matrices SiZ165 and Neural Basal System differentiation media

Biotechnology Laboratory PracticesKey Aspects:- Recombinant DNA technology & Genetic Engineering -> Purification of nucleic acid, molecular cloning via isolation, digestion, ligation & transformation in E. Coli, Bioinformatics analysis -> Screening of genetically modified food by operation in Polymerase Chain Reaction- Proteomics -> Extraction, concentration, purification of enzyme by gel permeation chromatography & activity detection by enzyme kinetics -> Practice in 2D Gel electrophoresis and western blot in studying properties of protein- Developmental Biology -> Conduct transgenesis in C. Elegans for the examination of embryogenesis -> Manipulation of toxins in effecting zebrafish development- Applied Immunology & Endocrinology -> Training in histochemistry, cultivation & primary cell culturing of Hematopoietic Bone Marrow Stem Cells -> Investigation of subpopulation of lymphocytes though flow cytometry, conduction of Immunoelectrophoretic & ELISA -> Manipulation in gene regulation on rat ovulation via hormonal mimicking compound, examination through histology & data analysis