Research fellow in the Infection and Immunity department working in the field of airway inflammation and studying the role of the neurotrophic factor neurturin in asthma Skills developed: - Mastery of cell culture with cell lines like A549, Calu3, BEAS2B and human primary cells (human airway epithelial cells) / Air-liquid interface culture - Viability and cytotoxicity assay on cells - Transepithelial electrical resistance measurement - Cytometric bead array assay - Flow cytometry (BD Fortessa and Novocyte Quanteon) for multipannel immune cells sorting and receptors identification - Enzyme-Linked Immunosorbent Assay (ELISA) for IgE, IgG1 and IgG2a evaluation - DNA/RNA extraction and purification - Mice genotyping - 16S rRNA extraction and purification + protocol elaboration - Polymerase Chain Reaction (PCR) / ReverseTranscription-PCR / qRT-PCR (16S rRNA) - Electrophoresis - Western blot - Introduced to library preparation and bacterial DNA sequencing - Histology : Use of the paraffin microtome and the citadel, hematoxylin and eosin staining of mice lungs samples - Work with mice : Obtention of the FELASA function A t o carry out minor procedures on animals, weighing, anesthesia, blood test, intranasal instillations, bronchoalveolar lavage fluid, introduced to Forced Oscillation Technique (FOT) with metacholine challenge, earmarking, euthanasia with CO2 chamber and cervical dislocation . - Budget management - Supplies management and ordering, price negociation with suppliers - Data analysis and scientific writing - Basic bioinformatic with first QIIME2 then R for sequencing data analysis - Scientific posters and talks elaboration and presentation - Good scientific practice knowledge

Second year of master internship for my Master thesis Subject: Effects of medium-chain triglycerides on the exercise capacity and the muscle metabolism.Skills developed: - Use of pH meter and solution preparation- Liquid Nitrogen Handling - Dissection of mice muscles under the microscope - Permeabilization and oxygen consumption measurement of the muscle fibers with an oxygraph (Oroboros oxygraph-2k software) - Real-Time Quantitative PCR - Work with mice , weighing, feeding different diets, blood sampling, spontaneous physical activity measurement, Effort and Endurance exercice test, anesthesia with isoflurane, extraction of gastrocnemius and quadriceps muscles

First year of Master's internshipSkills developed: - Antibodies testing by Western Blot - Bradford - Staining of megakaryocytes - Introduction to confocal microscopy and ImageJ software