Generation of artificial in-process controls for optical quality calibration, assay sensitivity, specificity and dynamic range definition.• Established protocols for nucleic acids and optical beads immobilization on glass surfaces. • Developed protocols for the synthesis of 3D hydrogels with embedded beads and biological material.• Performed Spatial Transcriptomics analysis on a variety of biological and artificial samples. • Synthetized and amplified fluorescent ssDNA via PCR/aPCR. • Extracted RNA from cells and FFPE tissue samples. • Hands-on experience with automated workflows (e.g. Hamilton Robotics). • Project management. • Data analysis and interpretation of Spatial Transcriptomics outputs.

Tissue sample processing for Spatial transcriptomics analysis; automated workflow testing for user-friendliness, usability, and more. • Processed tissue samples according to FFPE and FF protocols. • Performed manual and automated Spatial Transcriptomics analysis on cells, human and animal tissues. • Implemented and optimized automated workflows for user friendliness and technical efficiency (e.g. Hamilton Robotics). • Carried out cell culture batches production. • Data analysis and interpretation of Spatial Transcriptomics outputs.

• Assignments and exams correction and marking. • Students mentoring.

Synthesis of silica nanoparticles and peptides encapsulation for therapeutic purposes. • Established protocols for the synthesis of degradable peptide-loaded silica nanoparticles. • Performed material characterization via analytical techniques, such as DLS, TGA, HPLC, IR, UV-VIS, SEM. • Executed in-vitro biocompatibility tests on human fibroblasts. • Carried out lysosomal localization tests via ICC and confocal microscopy, as well as protein quantification via WB. • Data analysis and interpretation (e.g. OriginPro, ImageJ, Excel).

Laboratory technician at Hematology unit. • Processed human blood, CSF and urinary samples on automatic workflows for diagnostic purposes.