IndieBio Batch 14

- Polyketide synthase engineering for production of valuable chemicals in microbial hosts- Isoprenoid pathway engineering for production of valuable chemicals in microbial hosts- Bacterial host engineering for natural product biosynthesis platforms-Skills: molecular biology, biochemistry, organic synthesis, analytical chemistry, genetic engineering

-Participant via the Berkeley Innovative Solutions consulting program-Provided due diligence and go-to-market strategies for low-carbon energy solutions and clean energy infrastructure

- Biosynthesis of valuable chiral 3-hydroxyacids from carboxylic acid monomers using a seven enzyme in vitro cascade- Crystallography of acetoacetyl-CoA reductase- Skills: cloning, protein purification, biosynthesis, HPLC, LC/MS, NMR, crystallography, organic synthesis

- Determination of the effects of codon usage bias on the production of proteins integrated into the genome of Streptomyces venezuelae for metabolic engineering.- Skills: cloning, Streptomyces culturing, bacterial conjugation of phageintegration vectors, cell fluorescence measurements

- Identification of inhibitors for the acetoacetyl-CoA reductase enzyme from Burkholderia pseudomallei with the use of virtual screening methods.- Skills: cloning, protein purification, virtual ligand docking and screening

- Synthesis of polyubiquitin chains through the use of an azide-alkyne copper catalyzed click reaction at the site of unnaturally incorporated amino acids on ubiquitin moieties.- Skills: cloning, unnatural amino acid incorporation, protein purification, bioorthogonal chemistry