Luis Ramirez Hernandez Email & Phone Number

Los Angeles, Ca, Us

La Jolla, Ca, Us

San Francisco, California, Us

Investigating microbe-phage interactions and mechanisms of phage resistance (manuscript in work):• Helped develop novel methods for transformation and CRISPR-based gene-editing in gram-positive bacteria• Optimized protocols for RNA and HMW DNA extraction in Eggerthella lenta• Extracted and prepared samples for Illumina and Nanopore long-read sequencing• Helped prepare and image bacterial cell samples with TEM• Sample processing and extraction of RNA from mouse lung and cecal tissue• Optimization of low-throughput phage sensitivity screening• Designing, carrying out, and analyzing data from RNAseq experiments • Familiarity with sequencing data processing and analysis pipelines/software (ex: R, DESeq2, bowtie2)• Expertise with extraction, quantification, and overall handling of RNA samples• QC/QA of DNA and RNA using Agilent Tapestation 4200• Expertise in anaerobic culturing methods

Berkeley, Ca, Us

Project 3 - Phage engineering:• Successfully engineered bacteriophage as a gene delivery vector• Demonstrated the amenability for genome manipulation at multiple lociProject 2 - Functional genomics of Pseudomonas fluorescens:• Employed high-throughput functional genomics for the identification of genes involved in bacterial resistance to phage-tail like bacteriocins• Utilized a Dual-barcoded expression library (Dub-seq) to screen for resistant mutants • Handled sequencing data analysis, use of microplate reader, and mutant library generationProject 1 - Engineering Phage-tail like Bacteriocins (PTLBs):• Assisted in the development of a platform to engineer recombinant proteins with modified bactericidal properties • Constructed 6 new expression vectors and 6 new bacterial strains for said platform• Relevant skills: cloning, Gibson assembly and vector design, single-gene knockouts, site-directed mutagenesis, transformations, Sanger sequencing, protein purification/precipitation, UV and Hydrogen Peroxide PTLB Induction.

Berkeley, Ca, Us

• Free weekly 2-hour tutoring sessions for BSP students• Led weekly reviews, mock exams, and facilitated interactive learning activities for remote instruction• Helped over 20 students excel in one of the most difficult first-year chemistry course• Supported students from low-income, first-gen, latino, and other underrepresented backgrounds in STEM by providing academic and non-academic guidance and advice

Acceleration of phage therapy technologies:• Carried out experiments to optimize the efficiency of phage purification and extraction• Employed classical plaque-assays to evaluate the effect of lyophilization methods on the integrity and efficacy of phage extracts • Isolated, prepared, and sequenced hundreds of bacteriophage and bacterial isolates, including Cystic Fibrosis patient sputum samples, using Illumina NGS technologies• Gained expertise using MiSeq and NextSeq systems

Berkeley, Ca, Us

• Created transposon insertion mutants in gram-positive bacteria via delivery of transpososome complex.• Initiated designed of a platform to bioengineer bacteria that produce Phage tail-like Bacteriocins (PTLBs).

Berkeley, California, Us

• Ensure preservation of antique books, photographs, and maps. • Repair, mend and encapsulate items for circulation and archive rare collection items.

Berkeley, California, Us

• Taught elementary school science under the supervision of a teacher mentor• Developed and executed lesson plans• Encouraged student curiosity and engagement through sciences experiments and activities• Culminated with a presentation that introduced students to the field of Neuroscience.

Bachelor Of Arts - Ba, Molecular And Cell Biology

Education record