Luke Brownbridge Email & Phone Number

I project managed multiple LC-MS/MS based method validation and sample analysis studies (>30 studies as of January 2021). These studies covered small molecule, biomarker and peptide analytes.All work was carried out to either good laboratory practice or good clinical practice (for clinical trial sample analysis). Validations were carried out to FDA Bioanalytical Method Validation Guidance for Industry (2018) or EMA Guideline on bioanalytical method validation (2012).

Huntingdon, England, United Kingdom

I project managed multiple LC-MS/MS based method validation and sample analysis studies (>30 studies as of January 2021). These studies covered small molecule, biomarker and peptide analytes.All work was carried out to either good laboratory practice or good clinical practice (for clinical trial sample analysis). Validations were carried out to FDA Bioanalytical Method Validation Guidance for Industry (2018) or EMA Guideline on bioanalytical method validation (2012).

Manchester, United Kingdom

I worked within a lipidomic research group at the University of Manchester. I developed a mass spectrometry assay capable of quantifying skin ceramides from tape strips. I applied this assay to discern the seasonal variation of ceramides in normal and acne skin, and to test the efficacy of various topical treatments aimed at restoring ceramide levels. The project required me to contribute to papers as well as writing Methods, COSHH and risk assessments for internal use. From this project I have UPLC-MS/MS method development experience. The role included significant amounts of liquid chromatography and mass spectrometry problem solving. I passed this knowledge on via lab presentations or by training other members of staff to use this assay. I also carried out maintenance of the liquid chromatography systems and mass spectrometers as well as managed the laboratory consumables.

Sand Hutton York

I carried out offline Maldi-Tof/Tof (Bruker) discovery mass spectrometry to identify small peptides hormones from Diptera species. This involved alignment of cDNA sequenced reads into a expressed sequence tag dataset followed by MASCOT searches to identify the hormone of interest.

University Of York

My MSc in Bioscience Technologies covered many areas of modern molecular biology. The course was broken into 7 main scientific areas and 1 business based module, specifically; Molecular Biology, Biophysics, Proteomics, Genomics, Imaging and Flow Cytometry, Protein Production, Bioinformatics and Business and Leadership skills. The course also involved internal and external projects based around my chosen subject of proteomics:Internal ProjectValidation of a AQUA peptide quantification protocol for alpha amylase levels, this required; identification of a suitable AQUA peptide, assesment of the sensitivity and range of quantification, identification of peptides not derived from alpha amylase and not removed during extraction. Techniques used in this project include Maldi Tof CID Protein mass spectrometry, protein purification, bioinformatics and 2d gel electrophoresis and isoelectric focusing. External ProjectConducted offline Reverse Phase - MALDI ToF/ToF mass spectrometry of insect hormones coupled with bioassay to identify hormone effects on insect mating behaviour. This included extensive data analysis and interpretation with regard to experiments including Mascot searching, bioinformatics and calculation of false positive rates. Other techniques included insect dissection, protein extraction techniques.

Manchester, United Kingdom

I worked in a contract research organisation that carried out phase 1 and 2 clinical trials, specialising in treatments for respiratory and inflammatory diseases (specifically asthma and chronic obstructive pulmonary disease). This required me to work within the laboratory and with patients on clinical trials. My role included the following:- Assessing the severity of disease in patients using clinical methods such as: Spiriometry,Exhaled Nitric Oxide, Reversibility and Skin Prick testing for allergy. I explained the varioustesting methods and results to patients.- Processing both biopsy and sputum samples that could later be used for ELISA, Immunohistochemistry and RNA analysis.- Conducting differential cell counts on immune cells as part of patient screening for inclusion into clinical trials.- General Laboratory duties (sample storage, reagent preparation, electronic databasing of results etc).- Strict adherence to standard operating procedures.-All work was carried out to good clinical practice and good laboratory practice regulations.

Master, Bioscience Technologies

Biology, Bsc, 2:1

Education record