Luna Nascimento Vargas Email & Phone Number

Londrina, Pr, Br

Cambé, Paraná, Brazil

▪ I conduct genomic analyses for the development and validation of new molecular markers in soybean, corn, wheat, and sunflower crops.▪ I executed the validation protocols for DNA extraction, genotyping, and DNA sequencing.• I analyze genetic data generated through PCR and qPCR techniques, using TaqMan, MGB, and KASP assays.▪ I conduct in activities such as primer design, development of Standard Operating Procedures (SOPs), and field and greenhouse sampling.

Uberlândia, Minas Gerais, Brazil

▪ I conducted DNA extraction and genotyping processes on corn, soybean, and cotton seeds to assess homozygosity, detect transgenic events, identify adventitious presence, and quantify copy numbers.▪ I executed the genotyping using PCR and qPCR techniques, employing TaqMan assays for precise molecular analysis.▪ I collaborated with the team on a project to assess the viability of probes and primers, and we achieved a cost-saving of 300 thousand through the optimization of inactive laboratory inputs.▪ Experience in operating Biomek, Hamilton, Wellmate, Hydrocycler, and QuantStudio 5.

▪ I provided strategic consulting involving analysis of scientific research, market, and new products to discover new technologies related to the projects and challenges of the client.

Brasília, Distrito Federal, Brasil

▪ Investigated the DNA methylation profile using molecular biology techniques, including DNA extraction, primer design, PCR, agarose gel electrophoresis, molecular cloning, preparation of libraries for NGS, and microbiology.▪ Developed experiments on DNA number copies quantification and gene expression analysis, projects involving DNA and RNA extraction, and real-time PCR.▪ Coordinated four research projects, including the stages: financial resource management, methodology development, quotation and purchase of technical materials, biological material collection, experiments execution, molecular analysis, statistical data analysis, presentations in Portuguese and English, and reports for accountability.▪ Analyzed more than 1500 DNA sequences in silico, as Sanger sequencing results, in platforms and software (Quma and Chromas).▪ Led and coordinated the technical training on equipment (real-time PCR and thermal cycler), molecular biology techniques, and scientific experiments execution for the new researchers of the research group.▪ Executed projects involving assisted reproduction techniques in cattle, which include oocyte maturation, somatic cell nuclear transfer (cloning), micromanipulation, and in vitro embryonic/cell culture.▪ Organized and conducted with the team a practical course on molecular biology techniques for 20 professionals from the Ministry of Agriculture and Livestock (MAPA).▪ Analyzed data, organized the information, and developed a methodology for DNA methylation analysis in a single oocyte.▪ Developed and published scientific articles (currently 13 published articles) in high-impact journals in partnership with different research groups.

Brasília, Distrito Federal, Brasil

▪ Led and investigated research and development projects focused on gene expression analysis in the placenta, evaluating the gene expression difference between bovine animal clones using techniques such as RNA extraction and real-time PCR.▪ Developed and conducted the standardization of a research project focused on SNPs identification through genotyping by RFLP and research to identify RNAs using the Northern Blot technique.▪ Coordinated and conducted an introductory course on genetics and epigenetics for 40 undergraduate students, presented lectures with scientific results of my research project for postgraduate researchers, and presented results in 8 national congresses.▪ Analyzed in silico molecular data in international genetic databases (NCBI and UCSC), platforms, and software for DNA sequence analysis, primer design, statistical analysis, and writing texts and technical-scientific reports.

Québec, Quebec, Canada

▪ Investigated the spermatozoa DNA from bulls with different fertility to identify molecular markers for fertility using the ATAC-seq technique.▪ Conducted the samples from DNA extraction, PCR, and real-time PCR to prepare libraries for NGS.▪ Participated in technical discussions with researchers from 3 countries (Canada, China, and Brazil) and produced materials in English.

Brasília, Distrito Federal, Brasil

▪ Developed experiments using molecular biology techniques such as DNA extraction, PCR, electrophoresis, molecular cloning, microbiology, and DNA methylation analysis.▪ Collaborated with research experiments using animal reproduction biotechnologies such as in vitro embryo production and somatic cell nuclear transfer (cloning).

Uberlândia, Minas Gerais, Brasil

▪ Participated in the study and research group on stem cells, working with microvesicle isolation and purification, monolayer cell culture, three-dimensional cell culture (3D), and ELISA.▪ Contributed to the data collection of scientific articles for the elaboration/publication of a literature review article on mesenchymal stem cells.▪ Organized three scientific events in the biotechnology area, being responsible for structuring the schedule and managing logistics with the team.

Phd In Genetics And Biochemistry

▪ Epigenética do desenvolvimento inicial em bovinos e as técnicas de reprodução assistida

Master In Genetics And Biochemistry

▪ Perfil transcricional de genes relacionados com a reprogramação da metilação do DNA em placenta de bovinos clones

Bachelor'S Degree In Biotechnology

▪ Ação de exossomos derivados de células-tronco mesenquimais sobre células do câncer de próstata