Luz Marina Ceballos Diaz, Ph.D. Email and Phone Number
SUMMARY Detail-oriented bioprocess engineer with 10+ years of experience in DNA, RNA and protein expression and purification in R&D laboratory, and GMP manufacturing setting. My broad range of experiences range from expressing and purifying DNA, RNA, membrane and soluble proteins, refolding proteins from denaturing conditions, and developing analytical activity assays. I am always excited to learn something new and lend my expertise when I can. KEY TECHNICAL SKILLSGMP processing: single use; scale-up; column packing; buffer preparation; chromatography (Affinity, Membrane, Anion exchange and Cation exchange, hydrophobic); AKTA (Lab, pilot and process scale); Filtration (Ultrafiltration, Diafiltration, depth filtration, and viral filtration)Protein biochemistry: Recombinant protein expression in E. coli bacteria, Chromatography (affinity, gel filtration, and ion exchange) SDS-PAGE, western blot, ELISA, fluorescence, luminescence, UV-VIS, circular dichroism (CD), MALDI-TOF, Nuclear Magnetic Resonance (NMR)Molecular biology: Purification of DNA/RNA, Primer design, DNA sequencing, site-directed mutagenesis (Kunkel, Quick change, Q5), gel electrophoresis, PCR (colony, overlap, and error-prone), Cloning (Gibson, ligation independent, and restriction enzyme)Phage display: combinatorial library design, variant selection and optimizationComputer: Microsoft Office Suite (Word, Excel, PowerPoint), Adobe (Illustrator and Photoshop), LaTEX, Software (Unicorn, GraphPad Prism, PyMol, Chimera, SnapGene, Gen5)
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Senior Process EngineerIlluminaMunich, By, De
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Process Engineer 2Illumina Apr 2021 - PresentSan Diego, California, United States
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Process Engineer 2, Downstream MsatAbzena Feb 2020 - Apr 2021San Diego, California, United StatesPerform downstream tech transfer activities, scale up, writing master batch records and supporting the bioprocess technicians as they execute large-scale protein purification processes in a GMP environment with single-use technologies. -
Postdoctoral Research AssociateSanford Burnham Prebys Medical Discovery Institute Feb 2017 - Feb 2020La Jolla, CaIndependently led two projects to investigate the molecular mechanism that the plague-causing bacterium uses to inhibit the host immune system via a membrane associated proteinPerformed ELISA and NMR experiments, interpreted results, troubleshot, and stayed abreast with the latest scientific literature -
Graduate Student Researcher, Department Of Molecular Biology And BiochemistryUniversity Of California, Irvine Sep 2011 - Dec 2016Irvine, CaAdvisor: Prof. Gregory A. Weiss, PhDInitiated and lead two research projects: PROJECT 1: Characterizing a bacterial specific membrane protein that is required for iron transport and survival in Shigella Dysenteriae. PROJECT 2: Investigating the effects of vibrational forces on enzyme activity for chemical processing using biocatalysis . • Cloned, expressed and purified soluble and membrane associated recombinant proteins in E. coli cells• Use AKTA system (size exclusion, ion exchange, hydrophobic interaction, affinity chromatography) to purify milligram quantites of protein that can be used for protein characterization and kinetic studies. • Designed rational and combinatorial mutagenesis libraries to select for protein variants with desired characteristics ( thermal stability, solubility, higher affinity, specificity) by phage display.• Supervised and trained 4 undergraduate and 1 graduate student on molecular biology and biochemistry techniques -
Research TechnicianTufts University School Of Medicine Jul 2009 - Aug 2011Boston, Ma, United StatesAdvisor: Prof. F. Rob JacksonInvestigated how the LARK Drosophila melanogaster RNA-binding protein controls circadian behavior through translational regulation. • Generated radiolabeled RNA using in vitro transcription and investigated which region of the target RNA interacts with the RNA-binding protein by electrophoretic mobility shift assays (EMSA) -
Undergraduate Student ResearcherBoston University Jul 2007 - Jun 2009Boston, Ma, United StatesAdvisor: Prof. Kim McCallUndergraduate Researcher, Department of BiologyBoston University Advisor: Professor Kim McCall June 2007 – May 2009(Boston, MA)Performed a genetic screen to identify genes that function in non-caspase dependent programmed cell death in the Drosophila melanogaster ovary• Genetically crossed retro transposon mutants targeting genes on chromosome 2 and performed immunohistochemistry to observed ovarian cell death phenotypes by fluorescence and confocal microscopy• Maintained accurate written and electronic records of genetic mutants and their phenotype • Maintained over 1000 genetic fly stocks used by laboratory members.• Presented at the undergraduate research symposium and coauthor one presentation given at a national conference
Luz Marina Ceballos Diaz, Ph.D. Education Details
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University Of California, IrvineMolecular Biology & Biochemistry -
Biochemistry And Molecular Biology
Frequently Asked Questions about Luz Marina Ceballos Diaz, Ph.D.
What company does Luz Marina Ceballos Diaz, Ph.D. work for?
Luz Marina Ceballos Diaz, Ph.D. works for Illumina
What is Luz Marina Ceballos Diaz, Ph.D.'s role at the current company?
Luz Marina Ceballos Diaz, Ph.D.'s current role is Senior Process Engineer.
What schools did Luz Marina Ceballos Diaz, Ph.D. attend?
Luz Marina Ceballos Diaz, Ph.D. attended University Of California, Irvine, Boston University.
Who are Luz Marina Ceballos Diaz, Ph.D.'s colleagues?
Luz Marina Ceballos Diaz, Ph.D.'s colleagues are Edison Adler, Anthony Mai, Nate Polaske, Phd, Natasha Crake, Ryan Villamayor, Peng Liu, Akash M Iyer.
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