Mohammadali Mohammadpour Email & Phone Number

Mainz, Rhineland-Palatinate, Germany

Utrecht, Netherlands

Key Activities:• Characterization of engineered T cells using Multiparameter Flow Cytometry with complex staining panels• Phenotypic and functional analysis of T cells• Production, characterization, and titration of lentiviral vectors• Immune cell culture and isolation of immune cells from human blood• Genetic modification of immune cells by lentiviral transduction• Data analysis and result interpretation for scientific reports and group presentations• Training new team members and technicians in flow cytometry and related data analyses• Quality control of cell characterization data and ensuring the accurate analysis of generated results• Drafting working instructions for T-cell characterization• Designing and planning experiments• Presenting scientific results in group meetings• Involvement in CMC activities and related development studies• Reviewing experiments performed by team members in the electronic laboratory journal

Stockholm, Stockholm County, Sweden

The impact of hypoxia on the phenotype, function, and metabolism of anti-CD19 CAR T-CellsKey Activities:• PBMC isolation• Activation and expansion of T cells• Functional and phenotypic analysis of CAR-T cells using Flow Cytometry• Tumor Killing Assay • T cell stimulation with target cells or PMA and Ionomycin, followed by the measurement of cytokine repertoire and polyfunctionality• Relative gene expression analysis by RT-qPCR• RNA extraction and cDNA synthesis• qPCR primer designing and protocol optimization• Identification of key metabolic genes • Identification of an optimal housekeeping gene with stable expression in hypoxia• Retroviral vector production and transduction of T cells with retroviral vectors• Writing a scientific report of findings and presentation of results

Nijmegen, Gelderland, Netherlands

As an international ambassador at Radboudumc, I contributed to organizing online introductions and Q&A events for prospective international students.Key activities:• Supporting my colleagues during online events• Answering questions about studying at Radboud University• Sharing my experiences with prospective students• Assisting future students in familiarizing themselves with studying at Radboud University

Department Of Tumor Immunology, Rimls, Nijmegen, The Netherlands

Investigating cross-talk between myeloid-derived suppressor cells and dendritic cells to improve tumor susceptibility for immunotherapyKey activities:• PBMC isolation by ficoll density gradient centrifugation• Monocyte, Dendritic cell, B cells, and T cell isolation by Magnetic-Activated Cell Sorting (MACS)• Differentiation of Monocytes to Myeloid-Derived Suppressor Cells (MDSCs) and Monocyte-Derived Dendritic Cells (moDCs)• Phenotypic analysis of dendritic cells using Flow Cytometry• Functional analysis of dendritic cells by Mixed Lymphocyte Reaction (MLR) assays• Culture of primary/immune cells (such as Monocytes, DCs, and T cells)• Investigating the mode of cellular interactions by a transwell assay• 3D culture using collagen rings• Cytokine secretion analysis by ELISA• Writing a scientific report and poster presentation at the annual MMD symposium

Tehran, Iran

Responsibilities:• Helping students with their questions related to their course• Weekly problem-solving sessions • Helping with quizzes

Karaj County, Alborz Province, Iran

Master'S Degree, Molecular Mechanisms Of Disease

Bachelor Of Science - Bs, Cell/Cellular And Molecular Biology