Accompagnement de l'entreprise bYoRNA dans son développement scientifique

Non-coding RNAs, Differentiation and Development TeamStudy of the long non coding RNA XIST function in the haematopoietic system in humanSupervision of an intern (5 months)Technics and skills:Molecular and cellular biology : Genomic and plasmid DNA, and RNA isolation and purification, DNase Traitement, RT-PCRRNA-FISH, DNA-FISH, microscopy analysisTissue Culture – Primary (CD34+ cord blood cells) and Cell Lines (H9, H1, WiBr2, WiBR3 and K562). Differenciation of human embryonic stem cells into hematopoietic progenitors. CFU assay. Cells cloning. Flow CytometryGene Knocdonw/Gene editing technics : CRISPR-Cas9, CRISPR inhibitors, CRISPR-Bac.

Non-coding RNAs, Differentiation and Development TeamThesis Title: Study of the long non coding RNA XACT regulation and function in human during early embryonic development and in the haematopoietic systemResearch areas:Early human embryonic development - Stem cellsHaematopoietic system X chromosome inactivationLong non coding RNA Evolution LeukemiaTechnics and skills: Molecular and cellular biology : Genomic and plasmid DNA, and RNA isolation and purification, DNase Traitement, RT-PCRRNA-FISH, DNA-FISH, microscopy analysis Tissue Culture – Primary (CD34+ cord blood cells) and Cell Lines (H9, H1, WiBr2, WiBR3 and K562). Differenciation of human embryonic stem cells into hematopoietic progenitors. CFU assay. Cells cloning. Flow CytometryGene Knocdonw/Gene editing technics : CRISPR-Cas9, CRISPR inhibitors, CRISPR-Bac, siRNA, LNA-GapmeRs.Computationals : R studio, Python. RNA-seq analysis

Study of the expression of the long non coding RNA XACT in human embryonic stem cellsNon-coding RNAs, Differentiation and Development TeamMolecular and cellular biology : Embryonic stem cell culture, RNA FISH, RT-qPCR, Western Blot, siRNA, LNA-Gapmers

Etude des modifications post-traductionnelles de HP1 et de leurs impacts sur son interaction avec l'ARN et l'ADN.

Mise en place d'un protocole pour purifier un isoforme de la Whirline, protéine jouant un rôle dans l'audition. Etude de la cristallographie d'un des domaines de cette protéine.