Madelyn Shapiro Email and Phone Number
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Madelyn Shapiro is a Research Scientist III at Seattle Children's at Seattle Children's. She possess expertise in protein purification, pcr, biochemistry, microbiology, laboratory and 44 more skills.
Seattle Children'S
View- Website:
- seattlechildrens.org
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Research Scientist IiiSeattle Children'S Sep 2019 - PresentStudy of HIV pro-viral reservoirs in various cohorts. Development/implementation of NGS techniques to find HIV integration sites and examine the transcriptomics of HIV-infected cells. Comparison of methods to determine HIV integration sites in participants with low pro-viral loads. Discover HIV-infected cell proliferation pathways. -
Research Scientist IiiUniversity Of Washington Sep 2017 - Sep 2019Lab management and research applicable to wastewater treatment efficiency. Established lab-scale membrane biofilm reactors designed to oxidize ammonia efficiently. -
Research Scientist IiUniversity Of Washington Aug 2013 - Aug 2017SeattleIdentification and assignment of function to non-coding regulatory RNAs in Acinetobacter baumannii• Growth experiments and survival tests (nutrient starvation, desiccation) with Acinetobacter baumannii, RNA extraction, northern blots, cDNA library synthesis for RACE, qPCR and RNA seq.• Protein purification via Strep-Tactin/sepharose to study CsrA-RNA complex.• Maintenance of written and electronic lab records, collaboration of research efforts with colleagues.• Lab management duties such as supply orders and managing equipment repairs or replacements.• Hire, supervise, and mentor undergraduate lab assistantsAccomplishments:• ID and experimental verification of 50 non-coding RNAs (some of which bind proteins) in A. baumannii.• Determined pathway in which 9 non-coding RNAs function in A. baumannii. -
Laboratory TechnicianNatural Molecular Testing Corporation Nov 2012 - Apr 2013Renton, WaPerformed high throughput diagnostic DNA tests for personalized medicineTechnical experience• PCR and processing for testing on microarray chips and bead flow cytometry on up to 1000 clinical samples per day. Also involved repetitive, precise, manual pipetting.Other duties• Data processing and upload to patient database, SOP writing.
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Research AssistantUniversity Of Illinois At Urbana-Champaign Aug 2009 - Aug 2012Microbiology Department, University Of IllinoisStudied bacterial biotin synthesis using various in vivo, in vitro and bioinformatics methodsBacterial genetics • Plasmid and genomic manipulation in Escherichia coli and Bacillus subtilis • Enzyme functions tested in vivo in minimal mediumProtein biochemistry • Protein purification using Ni-NTA/His tags, ion exchange, and gel exclusion chromatography• Preparation of acyl-ACP substrates to test in vitro enzyme activitiesBioinformatics analyses • Sequence alignments (CLUSTELW, MUSCLE), phylogenetic trees, homologous structure modeling Other duties• Maintenance of written and electronic lab records, collaboration of research efforts with colleagues, presentations at lab meetings and conferences• Hire, supervise, and mentor undergraduate lab assistants• Mini lab rotation projects involved studying heme proteins in Thermus thermophiles, mechanisms of Saccharomyces yeast vacuole formation, and the structural intricacies of β-galactosidase• Teaching assistant (up to 4 classes of 25 students per semester in freshmen and sophomore-level Molecular and Cellular Biology courses)Accomplishments• Shapiro MM, Chakravartty V, Cronan JE (2012) Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin. PLoS ONE 7(11): e49440. doi:10.1371/journal.pone.0049440• Mapped out the regulatory importance of esterase activity in bacterial biotin synthesis -
Undergraduate Researcher In Michael Shiaris' Microbiology LabUmass Boston Jun 2007 - Jul 2009Studied environmental distribution of Saccharomyces yeasts Ecological studies • Field sample collection, selective yeast culture enrichment, microscopic characterization of budding and sporulating yeast, visual colony examination, PCR identification Other duties• Maintenance of written and electronic lab records, collaboration of research efforts with colleagues, presentations at lab meetings and conferencesAccomplishments• $4000 American Society for Microbiology (ASM) Undergraduate Research Fellowship, $1000 travel stipend to present results at ASM conference, 2008-2009• $500 University of Massachusetts Boston Honors Program Supply Grant, 2007• Honors degree in Biology earned with thesis.
Madelyn Shapiro Skills
Madelyn Shapiro Education Details
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Microbiology -
University Of Massachusetts BostonBiology
Frequently Asked Questions about Madelyn Shapiro
What company does Madelyn Shapiro work for?
Madelyn Shapiro works for Seattle Children's
What is Madelyn Shapiro's role at the current company?
Madelyn Shapiro's current role is Research Scientist III at Seattle Children's.
What is Madelyn Shapiro's email address?
Madelyn Shapiro's email address is madelyns@uw.edu
What is Madelyn Shapiro's direct phone number?
Madelyn Shapiro's direct phone number is (206) 543*****
What schools did Madelyn Shapiro attend?
Madelyn Shapiro attended University Of Illinois Urbana-Champaign, University Of Massachusetts Boston.
What skills is Madelyn Shapiro known for?
Madelyn Shapiro has skills like Protein Purification, Pcr, Biochemistry, Microbiology, Laboratory, Protein Expression, Flow Cytometry, Protein Chemistry, Microscopy, Western Blotting, Gel Electrophoresis, Dna Extraction.
Who are Madelyn Shapiro's colleagues?
Madelyn Shapiro's colleagues are Melissa Lawrence, Tiffany Miller, Lydia Li, Mie Yashiro, Henriques Amy, Madeline Pugar, Sabrina Register.
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Madelyn Shapiro
Clinical Research Assistant At Washington University In St. Louis School Of MedicineWashington, Dc -
Madelyn Shapiro, LCSW
Recent Graduate Student At Boston University'S School Of Social WorkBoston, Ma1trincoll.edu
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