Madelyn Shapiro

Madelyn Shapiro Email and Phone Number

Research Scientist III at Seattle Children's @ Seattle Children's
seattle, washington, united states
Madelyn Shapiro's Location
Seattle, Washington, United States, United States
Madelyn Shapiro's Contact Details

Madelyn Shapiro personal email

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About Madelyn Shapiro

Madelyn Shapiro is a Research Scientist III at Seattle Children's at Seattle Children's. She possess expertise in protein purification, pcr, biochemistry, microbiology, laboratory and 44 more skills.

Madelyn Shapiro's Current Company Details
Seattle Children's

Seattle Children'S

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Research Scientist III at Seattle Children's
seattle, washington, united states
Employees:
6071
Madelyn Shapiro Work Experience Details
  • Seattle Children'S
    Research Scientist Iii
    Seattle Children'S Sep 2019 - Present
    Study of HIV pro-viral reservoirs in various cohorts. Development/implementation of NGS techniques to find HIV integration sites and examine the transcriptomics of HIV-infected cells. Comparison of methods to determine HIV integration sites in participants with low pro-viral loads. Discover HIV-infected cell proliferation pathways.
  • University Of Washington
    Research Scientist Iii
    University Of Washington Sep 2017 - Sep 2019
    Lab management and research applicable to wastewater treatment efficiency. Established lab-scale membrane biofilm reactors designed to oxidize ammonia efficiently.
  • University Of Washington
    Research Scientist Ii
    University Of Washington Aug 2013 - Aug 2017
    Seattle
    Identification and assignment of function to non-coding regulatory RNAs in Acinetobacter baumannii• Growth experiments and survival tests (nutrient starvation, desiccation) with Acinetobacter baumannii, RNA extraction, northern blots, cDNA library synthesis for RACE, qPCR and RNA seq.• Protein purification via Strep-Tactin/sepharose to study CsrA-RNA complex.• Maintenance of written and electronic lab records, collaboration of research efforts with colleagues.• Lab management duties such as supply orders and managing equipment repairs or replacements.• Hire, supervise, and mentor undergraduate lab assistantsAccomplishments:• ID and experimental verification of 50 non-coding RNAs (some of which bind proteins) in A. baumannii.• Determined pathway in which 9 non-coding RNAs function in A. baumannii.
  • Natural Molecular Testing Corporation
    Laboratory Technician
    Natural Molecular Testing Corporation Nov 2012 - Apr 2013
    Renton, Wa
    Performed high throughput diagnostic DNA tests for personalized medicineTechnical experience• PCR and processing for testing on microarray chips and bead flow cytometry on up to 1000 clinical samples per day. Also involved repetitive, precise, manual pipetting.Other duties• Data processing and upload to patient database, SOP writing.
  • University Of Illinois At Urbana-Champaign
    Research Assistant
    University Of Illinois At Urbana-Champaign Aug 2009 - Aug 2012
    Microbiology Department, University Of Illinois
    Studied bacterial biotin synthesis using various in vivo, in vitro and bioinformatics methodsBacterial genetics • Plasmid and genomic manipulation in Escherichia coli and Bacillus subtilis • Enzyme functions tested in vivo in minimal mediumProtein biochemistry • Protein purification using Ni-NTA/His tags, ion exchange, and gel exclusion chromatography• Preparation of acyl-ACP substrates to test in vitro enzyme activitiesBioinformatics analyses • Sequence alignments (CLUSTELW, MUSCLE), phylogenetic trees, homologous structure modeling Other duties• Maintenance of written and electronic lab records, collaboration of research efforts with colleagues, presentations at lab meetings and conferences• Hire, supervise, and mentor undergraduate lab assistants• Mini lab rotation projects involved studying heme proteins in Thermus thermophiles, mechanisms of Saccharomyces yeast vacuole formation, and the structural intricacies of β-galactosidase• Teaching assistant (up to 4 classes of 25 students per semester in freshmen and sophomore-level Molecular and Cellular Biology courses)Accomplishments• Shapiro MM, Chakravartty V, Cronan JE (2012) Remarkable Diversity in the Enzymes Catalyzing the Last Step in Synthesis of the Pimelate Moiety of Biotin. PLoS ONE 7(11): e49440. doi:10.1371/journal.pone.0049440• Mapped out the regulatory importance of esterase activity in bacterial biotin synthesis
  • Umass Boston
    Undergraduate Researcher In Michael Shiaris' Microbiology Lab
    Umass Boston Jun 2007 - Jul 2009
    Studied environmental distribution of Saccharomyces yeasts Ecological studies • Field sample collection, selective yeast culture enrichment, microscopic characterization of budding and sporulating yeast, visual colony examination, PCR identification Other duties• Maintenance of written and electronic lab records, collaboration of research efforts with colleagues, presentations at lab meetings and conferencesAccomplishments• $4000 American Society for Microbiology (ASM) Undergraduate Research Fellowship, $1000 travel stipend to present results at ASM conference, 2008-2009• $500 University of Massachusetts Boston Honors Program Supply Grant, 2007• Honors degree in Biology earned with thesis.

Madelyn Shapiro Skills

Protein Purification Pcr Biochemistry Microbiology Laboratory Protein Expression Flow Cytometry Protein Chemistry Microscopy Western Blotting Gel Electrophoresis Dna Extraction Chromatography Molecular Genetics Data Analysis Mass Spectrometry Rt Pcr Qpcr Polymerase Chain Reaction Microsoft Office Bacterial Culture Fplc Structural Bioinformatics Microbial Ecology Record Keeping Data Presentation Grant Writing Immunohistochemistry Molecular Evolution Experimental Design Microsoft Word Microsoft Excel Powerpoint Cloning Aseptic Technique Site Directed Mutagenesis Bacterial Transformation Cell Lysis Akta Uv/vis Troubleshooting Blast Ion Exchange Recombineering In Vivo Rna Isolation Rna Extraction Rnaseq Cdna Library Prep

Madelyn Shapiro Education Details

Frequently Asked Questions about Madelyn Shapiro

What company does Madelyn Shapiro work for?

Madelyn Shapiro works for Seattle Children's

What is Madelyn Shapiro's role at the current company?

Madelyn Shapiro's current role is Research Scientist III at Seattle Children's.

What is Madelyn Shapiro's email address?

Madelyn Shapiro's email address is madelyns@uw.edu

What is Madelyn Shapiro's direct phone number?

Madelyn Shapiro's direct phone number is (206) 543*****

What schools did Madelyn Shapiro attend?

Madelyn Shapiro attended University Of Illinois Urbana-Champaign, University Of Massachusetts Boston.

What skills is Madelyn Shapiro known for?

Madelyn Shapiro has skills like Protein Purification, Pcr, Biochemistry, Microbiology, Laboratory, Protein Expression, Flow Cytometry, Protein Chemistry, Microscopy, Western Blotting, Gel Electrophoresis, Dna Extraction.

Who are Madelyn Shapiro's colleagues?

Madelyn Shapiro's colleagues are Melissa Lawrence, Tiffany Miller, Lydia Li, Mie Yashiro, Henriques Amy, Madeline Pugar, Sabrina Register.

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