Madhuri Tilva Email & Phone Number

Burlington, Massachusetts, United States

Cambridge, Massachusetts, Us

-Regularly purified novel protein products from microgram to gram scale using AKTA FPLC systems, Waters Preparative HPLC, and TFF-Scaled up and produced gram-scale polymer-based drug substance to support formulation development, stability, and toxicology studies -Purified and characterized materials (Reagents, Antibodies, Polymers) for research reagents, in-vivo/ex-vivo, PK, ADME, and tox studies-Established and managed protein product inventory system for all research activities -Characterized product material using Octet, Biacore, DSF and HPLC (SEC, RP, IEX, HIC)-Developed an octet-based method for quantifying drug-antibody-ratio in support of antibody-drug-conjugate program development -Performed accelerated stability screening campaign to identify potential leads for therapeutic development using HPLC, Biacore, and Octet instruments-Cross-trained junior scientist

Cambridge, Ma, Us

Tarrytown, New York, Us

• Purified numerous tagged proteins, fusion proteins and antibodies with different structure for the R&D pipeline using conventional chromatography methods (Affinity and Size exclusion)• Operated purification equipment of GE AKTA platforms using UNICORN software: AKTA Avant, AKTA Explorer, and AKTA pure • Characterized reagents and antibodies using SEC chromatography, SDS PAGE electrophoresis and western blotting• Conjugated reagents and antibodies with biotin• Performed complex data analysis and Prepared presentation slides for characterization and documentation of reagents and antibodies purified• Managed, Organized and Dispensed reagent and antibodies inventory with real-time tracking through a LIMS-based system• Experience with liquid-handling automation equipment of Hamilton Microlab Star, Notebook keeping using (ELN), LIMS and SharePoint

Irving, Texas, Us

• Developed and executed experiments to study microvesicle and their biomarkers • Developed /Optimized nucleic acid-protein immunopercipitation• Studied Nucleic acids-protein binding/interaction using Aptamer immunoprecipitation, SBED conjugation, qPCR, nucleic acids-ELISA, Blotting and other techniques • Maintain inventory of key reagents and lab supplies

Irving, Texas, Us

• Managed and executed projects with minimal supervision • Developed/Optimized IP protocol for isolating interested protein • Develop/optimized conjugation chemistry of antibodies to various bead types • Studied protein binding/interaction and analyzed protein using SDS-Page, Western Blots, ELISA, Luminex, dotblot,DMFA,DLS and IP• Optimized/implemented downstream QC protocols for purified antibodies ( buffer exchange, SDS-Page and DLS)• Developed/Optimized ELISA (QC) of critical antibodies for IHC testing • Produced multiple lysates and generated paraffin embedded cell blocks from various cell line • Maintain TC facility, keep inventory of reagents and Lab supplies • Worked in a collaborative team effort with other team members, good team player and managed multiple projects simultaneously

Irving, Texas, Us

• Produced antibodies from Hybridoma cell line using Bioreactor P1000 • Exosome production and purification from various cell lines• Analyzed data of ELISA and BCA to characterize and quantify the protein• Purified protein using FPLC AKTA avant • Worked in a collaborative team effort with other team members• Aseptic Techniques, Luminar flow hood, ELISA, Bradford assay and other techniques were used

University Park, Pa, Us

• Worked on Csr global regulatory system of E.coli , RNA binding protein (CsrA )• Created 8 Strains of PhoR , UspA , MreB and CytR gene with CsrA + and CsrA – mutant, to study -how each gene regulates in presences of CsrA , also site directed mutagenesis was performed on RpoE Strains to understand critical gene of CsrA• Analyzed data of β-gal assay to characterize and quantify the enzyme activity from these strains. • Worked in a collaborative team effort with graduate and post-doctoral students.• PCR, Cloning, P-1transduction, Biorad assay, B-Galactosidase assay and other techniques were used

University Park, Pa, Us

• Helped with graduate students’ ongoing research ( Geobacter membrane protein OmcB , OmcZ,OmcE and OmcS ) • Verified insertion of the genes, determine optimal conditions for protein synthesis and protein purification using various techniques • Characterizing qualitative and quantitative assessments of various purified proteins• Miniprep, Cloning, Digestion, Transformation, Blotting, Sonication, Nickel- Column Chromatography, Bradford assay and other technique were used