Maen Sarhan, Phd Email & Phone Number

California, United States

South San Francisco, California, US

South San Francisco, California, US

South San Francisco, California, US

• Specialize in bridging the gap between Research & Development and Operations to streamline the implementation of large-scale processes for DNA-based product manufacturing.Key Highlights:
• Managing design assurance and quality engineering activities before production, ensuring seamless alignment with the product lifecycle development.
• Orchestrating cross-functional updates within an ISO 13485 certified manufacturing environment. This involves meticulous planning and execution of verification and validation strategies, conducting comprehensive risk.
• Supporting Corrective and Preventive Actions (CAPA) and overseeing production incident investigations by employing methodical planning, execution, and rigorous review processes.
• Communicating complex experimental findings through articulate verbal presentations and concise written reports.

• Applying a broad knowledge of assay development, data analytics, and project management to improve first pass yield on production grade assays that are based on customized liquid handling instruments and next.
• Maintaining high first pass yield for production grade assays by continuous monitoring of batch records using SQL based repositories.
• Interfacing with reagent manufacturing in troubleshooting underperforming reagents.
• Owned all aspects of tech transfer including training and monitoring post-release.
• Planning, organizing, and executing lab capacity scaling to maintain lab turnaround time
• Developing improvements to assays based on the latest literature findings

San Francisco, California, US

• Generation of pico-liter sized reaction vessels for high-throughput screening.
• Cell-free expression of membrane proteins in microfluidically generated liposomes.
• Developing ultra high throughput screening assays using RNA aptamers or fluorescently labelled substrates.
• Design and fabrication of microfluidic circuits using soft lithography.

South San Francisco, CA, US

Supporting Alliance Management with BARDA interactions

Chevy Chase, Maryland, US

• Biophysical characterization of ion channels and transporter proteins.
• Expressed recombinant membrane proteins in mammalian and bacterial systems.
• Purified, to homogeneity, proteins using BioRad Duoflow and AKTA FPLC of membrane proteins in detergents and amphipoles. Used IMAC, SEC, IAC, and IEX.
• Functionally characterized activity of protein in both HEK and E.coli with whole cell patch clamp electrophysiology, florescence, and radioactive uptake.
• Screened for crystallization of proteins using liquid handling robots in hanging drop, sitting drop, or lipidic cubic phase.
• Examined protein-ligand and protein-protein interactions using ITC.

Vancouver, British Columbia, CA

• Examined the molecular mechanism by which the human cardiac sodium channel is regulated by cytosolic calcium.
• Expressed (in E.coli) and purified soluble regions of a membrane protein and the eukaryotic calcium sensor calmodulin with gravity flow and AKTA FPLC. Used IMAC, SEC, IEX, RPC, and HIC.
• Whole cell patch clamp electrophysiology of transfected HEK cells expressing the human cardiac sodium channel was performed with various concentrations of intracellular calcium. Cell surface expression of cd4, or.
• Biophysical characterization of physiological mutants from patients to guide pharmacological treatments for patients in hospital.
• Binding thermodynamics were determined by ITC between calmodulin and soluble ion channel subunits or methyltransferases and histones.
• High resolution crystal structure of a protein complex was obtained by diffracting crystals at the Canadian light source (PDB 4DJC).

Doctor Of Philosophy (Phd), Pharmacology

Bachelor'S Degree, Pharmacology

Joint Major: Business, Biochemistry, And Molecular Biology