Maen Sarhan, Phd Email & Phone Number

California, United States

South San Francisco, California, Us

South San Francisco, California, Us

South San Francisco, California, Us

Specialize in bridging the gap between Research & Development and Operations to streamline the implementation of large-scale processes for DNA-based product manufacturing.Key Highlights:• Managing design assurance and quality engineering activities before production, ensuring seamless alignment with the product lifecycle development.• Orchestrating cross-functional updates within an ISO 13485 certified manufacturing environment. This involves meticulous planning and execution of verification and validation strategies, conducting comprehensive risk analyses, and facilitating formal design reviews.• Supporting Corrective and Preventive Actions (CAPA) and overseeing production incident investigations by employing methodical planning, execution, and rigorous review processes.• Communicating complex experimental findings through articulate verbal presentations and concise written reports.

Applying a broad knowledge of assay development, data analytics, and project management to improve first pass yield on production grade assays that are based on customized liquid handling instruments and next generation sequencing. • Maintaining high first pass yield for production grade assays by continuous monitoring of batch records using SQL based repositories.• Interfacing with reagent manufacturing in troubleshooting underperforming reagents.• Owned all aspects of tech transfer including training and monitoring post-release. • Planning, organizing, and executing lab capacity scaling to maintain lab turnaround time• Developing improvements to assays based on the latest literature findings• Independent design of experimental plans including performing studies, analyzing large data sets, and reporting study results and recommendations both verbally and in written formats• Worked with software teams to develop workflow support for CLIA lab operators.

San Francisco, California, Us

Generation of pico-liter sized reaction vessels for high-throughput screening.• Cell-free expression of membrane proteins in microfluidically generated liposomes. • Developing ultra high throughput screening assays using RNA aptamers or fluorescently labelled substrates. • Design and fabrication of microfluidic circuits using soft lithography.

South San Francisco, Ca, Us

Supporting Alliance Management with BARDA interactions

Chevy Chase, Maryland, Us

Biophysical characterization of ion channels and transporter proteins.• Expressed recombinant membrane proteins in mammalian and bacterial systems.• Purified, to homogeneity, proteins using BioRad Duoflow and AKTA FPLC of membrane proteins in detergents and amphipoles. Used IMAC, SEC, IAC, and IEX. • Functionally characterized activity of protein in both HEK and E.coli with whole cell patch clamp electrophysiology, florescence , and radioactive uptake.• Screened for crystallization of proteins using liquid handling robots in hanging drop, sitting drop, or lipidic cubic phase. • Examined protein-ligand and protein-protein interactions using ITC.• Diffracted membrane protein crystals at high intensity beamline sites, ALS and SSRL, and with an in-house X-ray diffractor.

Vancouver, British Columbia, Ca

Examined the molecular mechanism by which the human cardiac sodium channel is regulated by cytosolic calcium.• Expressed (in E.coli) and purified soluble regions of a membrane protein and the eukaryotic calcium sensor calmodulin with gravity flow and AKTA FPLC. Used IMAC, SEC, IEX, RPC, and HIC.• Whole cell patch clamp electrophysiology of transfected HEK cells expressing the human cardiac sodium channel was performed with various concentrations of intracellular calcium. Cell surface expression of cd4, or intracellular eGFP was used as a marker of transfection.• Biophysical characterization of physiological mutants from patients to guide pharmacological treatments for patients in hospital.• Binding thermodynamics were determined by ITC between calmodulin and soluble ion channel subunits or methyltransferases and histones. • High resolution crystal structure of a protein complex was obtained by diffracting crystals at the Canadian light source (PDB 4DJC).• PCR, restriction free cloning, and site directed mutagenesis were used to prepare plasmids.

Doctor Of Philosophy (Phd), Pharmacology

Bachelor'S Degree, Pharmacology

Joint Major: Business, Biochemistry, And Molecular Biology