Mahadesh Prasad A.J, Phd personal email
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I am committed, reliable, a biomedical scientist with excellent hands-on experience in vaccine development, molecular immunology, and Hematopoietic stem cell biology. I am a driven scientist, motivated to develop creative research designs and protocols that provide answers to complex biological challenges. My area of research is mainly focused on developing the vaccines for SARS-CoV-2 and ZIKA virus using Yellow fever-17D backbone. Additionally, I am extensively involved in understanding the molecular events involved in viral associated B- cell transformation and elucidating the role of early transcription factors in B- cell maturation and in formation of B- cell malignancies during early hematopoietic stem cell maturation using cell culture and mouse model. I am a confident, excellent project manager playing an important role in several independent projects resulting in publication in high-impact journals such as Nature, Blood, Journal of Virology, PLOS Pathogen and Oncotarget, etc. In addition to managing projects, I am continuously involved in grant application writing, manuscript writing, reviewing and supervising Ph.D. students, project students, and technical staff. As an eager team player I can bring in the following analytical skills:vaccine design, cell culture, primary immune cell culture and isolation, cloning, WB, qRT PCR, ChIP Seq, multi-color flowcytometry, confocal and fluorescence imaging, IHF, IP, GST pull-down, virus infection studies, chemical synthesis of novel compounds.
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Executive Research ConsultantMedxury ® Jan 2024 - PresentIndia -
Research And Development ConsultantBtl India Private Ltd Jul 2022 - PresentBengaluru,India And Czech Republic -
Professor And In Charge Of Mahajana Research FoundationDepartment Of Biochemistry, School Of Life Sciences, Pbmm Pg Centre, Affiliated To Uom Dec 2021 - PresentMysore, Karnataka, India
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Senior Vaccine ExpertFhi 360 Dec 2021 - Dec 2022United States -
Scientific ResearcherKu Leuven Mar 2019 - Dec 2021Belgium -
Serb-Dst Principal Investigator /Assistant ProfessorDepartment Of Biochemistry, Sbrr Mahajana Pg Centre, Affiliated To University Of Mysore Aug 2015 - Mar 2019MysoreMy main responsibility includes• Leading Independent research project to understand “DNA damage response pathway in Burkitt’s lymphoma”. Lecturing MSc Biochemistry students. • Developing and optimisation of new techniques, working in close collaboration with hospitals and external partners to obtain clinical samples/ clinical data. • Project supervision, project management, project plans, SOP’s and lab management. Supervising technical staff, project students and PhD students. • Manuscript writing, grant application writing, editing, reviewing and curriculum design. Administrative duties such as board of studies, board of examination, admissions and counseling. Lab equipment’s purchase and negotiation.
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Postdoctoral Scientist✔Immunologist ✔Leukeamia Research✔In-Vivo Models✔Project ManagmentLinkoping University Feb 2013 - Jul 2015Linkoping, SwedenWorked extensively on understanding the role of Early Transcription Factors (ETF) in early hematopoietic stem cells maturation and how altered dose of ETF results in B- cell leukeamia formation. • Hands on experience in handling and managing laboratory Haploinsufficient mouse models.• Performed cellular and molecular biology experiments using mouse models and animal cell lines.• Handling and storage of serum, bone marrow, liver and spleen samples.• Developed a rapid experimental protocol to make Chromatin immuno precipitation (ChIP) library.• Involved in study design for Proximity Ligation Assay (PLA) protocol.• Worked closely with other group leaders to design a new project on mapping Intractomes of crucial transcription factors in B- cell development using BIO-ID method. • Curate and maintain detailed laboratory records • Published original research article in Blood and published a review article in the blood as well. -
Postdoctoral FellowUniversity Of Pennsylvania School Of Medicine Nov 2010 - Dec 2012Philadelphia, UsaMy postdoctoral research was mainly focused on elucidating molecular mechanism involved in B- cell transformation during Epstein Barr- Virus (EBV) infection.• Designed a novel project to explore the importance of EBV onco protein EBV nuclear antigen 3C (EBNA3C) in the formation of B- cell malignancies via inducing DNA damage response. • Performed EBV virus preparation and infection studies. • Optimized the study protocol to infect human peripheral blood mononuclear cells with EBV virus.• Generated number of different mutations in full length EBNA3C oncoprotein to identify the importance of different regions in B- cell transformation. • Performed flow analysis and proteomics studies on mammalian cells to understand transformation process during EBV infection. • Supervised project students for master thesis. • Reviewed manuscripts from major science journals. • Published research articles in high impact journals -
Visiting Researcher/Postdoctoral FellowUniversity Of Leipzig Aug 2009 - Nov 2010Leipzig Area, GermanyAfter submitting my PhD thesis to University of Mysore in 2009 June, I was invited as a visiting researcher to investigate the role of clinically important serum enzymes in development of cardiovascular diseases in domestic animals.More often domestic animals suffer heart related disorders which leads to prolonged illness and may even death. My project was mainly focused on evaluation of level of antioxidant enzymes particularly, Paraoxonase1 (PON1) enzyme in Canine suffering from Dilated Cardiomyopathy (DCM). PON1 is high-density lipoprotein associated esterase capable of hydrolyzing various organophosphates and protects low-density lipoprotein peroxidation. PON1 is essentially involved in prevention of cardiovascular diseases and the level is significantly decreased in myocardial infarction, liver disease and in oxidative stress. Our results form evaluation of serum samples from DCM with a varied illness showed that PON1 activity was significantly decreased in stage IV DCM, compared to healthy canine and stage II and III. -
Phd CandidateUniversity Of Mysore Aug 2006 - Jul 2009MysoreCompleted PhD in Biochemistry under supervision of Prof. Cletus JM D' Souza and Prof. Kemparaju.K, thesis entitled Substrate specificity of Paraoxonase1 (PON1) and their implication in cardiovascular diseases My thesis work represents the rapid method of purification of PON1 enzyme by advanced methods. My thesis work was mainly focused on identifying and synthesising novel potential atherogenic molecules such as fatty acid esters and cholesterol esters. These synthesised substrates were screened for substrate specificity of PON1. We extensively screened natural substrates such as Penicillin, Vitamin A as a substrate for Paraoxonase. During my PhD work we had collaboration with number of software firms to obtain clinical samples from their employees. We assessed the clinical samples form south Indians to monitor the susceptibility towards cardiovascular diseases
Mahadesh Prasad A.J, Phd Skills
Mahadesh Prasad A.J, Phd Education Details
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Biochemistry -
Biochemistry -
Govt Science College, HassanFirst
Frequently Asked Questions about Mahadesh Prasad A.J, Phd
What company does Mahadesh Prasad A.J, Phd work for?
Mahadesh Prasad A.J, Phd works for Medxury ®
What is Mahadesh Prasad A.J, Phd's role at the current company?
Mahadesh Prasad A.J, Phd's current role is Professor.
What is Mahadesh Prasad A.J, Phd's email address?
Mahadesh Prasad A.J, Phd's email address is aj****@****ail.com
What schools did Mahadesh Prasad A.J, Phd attend?
Mahadesh Prasad A.J, Phd attended University Of Mysore, University Of Mysore, Govt Science College, Hassan, University Of Pennsylvania, University Of Pennsylvania.
What skills is Mahadesh Prasad A.J, Phd known for?
Mahadesh Prasad A.J, Phd has skills like Cell Culture, Pcr, Western Blotting, Real Time Pcr, Transfection, Protein Purification, Immunology, Immunohistochemistry, Confocal Microscopy, Virus Culture, Virus Induction, Dna Damage.
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