Anjali Malik, Ph.D. Email & Phone Number

Scientific researcher with over 9 years of working experience in reputed research & academic institutions with a major focus on:* Cloning, Expression and purification of proteins from E.coli and mammalian expression systems.* Biochemical and biophysical characterization of proteins* Structural determination of proteins and structure-guided drug discovery. Focus of my research is to structurally and functionally characterize the growth factors belonging to TGF-beta family in-order to dig out the essential molecular and structural informations which would further be harnessed for therapeutic purposes. My research is primarily facilitated by a wide range of molecular biology, biochemistry and structure biology tools and techniques and I possess high level proficiency in the following skills-*Molecular Biology; Recombinant DNA construction l Gene cloning l PCR l DNA extraction and Purification .*Cell culture; Bacterial and mammalian transformation & transfection l Generation of transient and stable cell lines l E.coli culture l Protein expression in bacterial and mammalian systems.*Biochemistry; Protein purification l Chromatography techniques - IMAC, Ion exchange, SEC, AKTA l SDS-PAGE l Western blot l Agarose gel electrophoresis l Enzymatic activity assays- FRET & spectroscopy l Small molecule inhibitor/drug Screening.*Biophysical Methods; ITC (Isothermal Titration Calorimetry) l SPR (Surface Plasmon Resonance) l CD (Circular Dichroism ) l DSC (Differential Scanning Calorimetry).*Structural Biology; Protein crystallization l Crystal mounting and diffraction l Data collection l Structural solution and Refinement l Structural analysis.*Antibody purification and SPR based epitope mapping*Software Proficiency; PyMol, WinCoot, CCP4i suit, Phenix suit, BioRender, MS office tools.