Manali P. Email & Phone Number

Investigate the underlying mechanisms of stem cell aging to improve current regenerative therapies. ̶ Explore mechanisms underlying stem cell aging and exhaustion. ̶ Developing comprehensive protocols for reprogramming and neuronal differentiation of human skin and cardiac fibroblasts. ̶ Expertly managed and oversaw the successful completion of multiple research projects under time-sensitive conditions, including securing grants.̶ Mentored and supported the professional growth of research trainees.

Philadelphia, Pennsylvania, United States

Investigated novel mechanisms underlying somatic cell senescence and tumor suppression. ̶ Discovered the pivotal role of lncRNA H19 in modulating chromatin and inducing cellular senescence, significantly advancing our knowledge in the field. ̶ Designed innovative isolation and functional assays to more comprehensively analyze non-coding RNAs. ̶ Contributed to the dissemination of research findings by authoring technical reports and delivering impactful presentations to both scientific and non-scientific audiences.Better characterize the effects of rapamycin on potency and lifespan of in vitro expanded human mesenchymal stem cells.̶ Conducted a study to determine the impact of dosage-controlled rapamycin on the preservation of stemness and differentiation capabilities of in vitro expanded human mesenchymal stem cells. ̶ Demonstrated exceptional project management and collaboration skills in a laboratory setting, resulting in the successful development of methods that are both reproducible and transferable to other research environments.

Philadelphia, Pennsylvania, United States

Determined cellular mechanisms underlying a novel lifespan-enhancing intervention.̶ Investigated the impact of rapamycin and methionine restriction on senescence-associated changes in chromatin architecture through the study of protective mechanisms.̶ Designed and executed ATAC-seq assay protocols to evaluate the genome-wide accessibility of chromatin.̶ Analyzed and visualized data using R programming, providing critical insights into the project outcomes.Assisted in a randomized exploratory skin aging clinical trial. ̶ Conducted an in-depth analysis of gene expression and immune profiles in patients treated with topical rapamycin formulations, providing valuable insights into the therapeutic effects of the treatment.̶ Demonstrated expertise in handling and processing patient samples, ensuring high-quality data collection and preservation.̶ Collaborated effectively with scientists, clinical staff, and physicians, contributing to the success of the trial.

Characterized technically challenging recombinant Trichoderma virens proteins. ̶ Expressed, purified, and crystallized the C3PO complex with high yield and purity.̶ Employed X-ray crystallography to study the structural details of the C3PO-RISC interactions, providing a deeper understanding of the underlying biological mechanisms.̶ Purified the recombinant proteins with precision using a combination of HPLC, IEX, and size exclusion chromatography techniques, ensuring high-quality protein samples.

Mumbai, Maharashtra

• Assisted collaborative research aimed at understanding cancer metastasis from an ecological perspective.• Developed key phylogenetic analysis models critical to investigating the evolutionary and ecological impact on pro-tumor genes.

Doctor Of Philosophy - Phd, Biochemistry And Molecular Biology

Activities and Societies: Biomedical Sciences Graduate Student Association (BSGSA) member, VP of Community Service and Scientific Outreach.

Master Of Science - Ms, Molecular Biology And Genetics

Activities and Societies: Biomedical Sciences Graduate Student Association (BSGSA) member, VP of Community Service and Scientific Outreach.

Master Of Technology - Mtech, Biotechnology