Manasa Addagarla Email & Phone Number

Wayne, Pennsylvania, United States

Managed and performed gene deletions in 'Haloferax volcanii' to reduce His-tag interference optimizing protein purification.Investigated metabolic, oxidative-stress response of halophilic extremophiles resulting in promising biofuel applications.Conducted comprehensive evaluations of mutant strains through various assays and analyses.Conducted PCR, colony PCR experiments to amplify DNA sequences for further analyses.Conducted cPCRs to screen and identify clones with desired genetic characters.Assisted in comprehensive evaluations of mutant strains through various assays and analyses.Proficient in running and analyzing electrophoresis gels and visualizing nucleic acids and proteins.Analyzed gel results to assess the size, and quality of DNA fragments and proteins.Proficient in plating, streaking, maintenance plates, creating isolation plates and identification of microbial stains.Ran Western Blot experiments to analyze protein expression levels and post-translational modifications.Executed protein purification protocols, including transformation techniques and chromatography.Ensured proper handling and storage of samples to maintain sample integrity.

Gainesville, Florida, United States

Organized departmental initiatives and programs, while coordinating with a team of staff, students, and faculty from more than one University departments.• Conduct presentations and tours of Engagement offices to students, staff and/or patrons.• Serve as pillar chairs and strengthened the Center’s motto through scheduling interactive workshops.

• Acted as the Multimedia coordinator for a nationally ranked Early Arrival Program, providing media content and marketing.

• Tutored university level Chemistry, Calculus, Physics, and Biology.• Raising scores up to 2 letter grades, structured course material and practice sets tailored toward individual clients needs.• 50+ student clientele per class, organizing review session, 1-1 sessions and course videos.

Gainesville, Florida, United States

PGENeS LabProgram In Genetics and Epidemiological Symptoms of Neuropsychiatric DisordersInvestigated the genetic and epidemiological factors of neuropsychiatric disorders- Tourette’s syndrome, OCD, Hoarding disorder.• Data Mining and management of large-sized patient data into medical databases.• Assisted in the management of analyzing patient genetic data/ drug history.• Using Biostatistics and health psychology to integrate data into RedCap databases.• Analyzed/brainstormed research design for TDT studies.

• Managed social media accounts, creating graphic design posters and marketing towards the University student body. • Facilitated the pairing of nanoscale research labs with students via database.• Operated and worked with the Nanoscale research facility staff to engage students in Nanoscale research at a university-wide level.• Worked closely with researcher professionals and industry liaisons to bring Nanoscale technology to student career fronts.

• Pitch the importance of accessible global education and hygiene to investors and sponsors nationwide.• Advocate for equity and inclusion for female representation and women’s education.• Orchestrated community events to advocate for girl’s global education and hygiene

Gainesville, Florida, United States

• Aided in the technological research and design of prosthetic assistive devices for disabled children.• Promoted protocols to efficiently improve assistive devices, emphasizing on sustainability and broadening device usage.

Bachelor'S Degree, Biotechnology And Biomolecular Engineering

Bachelor'S Degree, Biotechnology

Activities and Societies: Nanoscience and Nanotechnology Student Council, She's the First, Generational Relief in Prosthetics, Knack.