Biochem. and Biotech. University of Milan Study of sphingolipid metabolism at the level of the membrane, and the distribution of different molecular species of particular sphingolipids that can alter membrane geometry, in normal and cancer cells and in some neuronal diseases.

Study of altered metabolism of gangliosides in Salla disease

provided from Italian Ministry of Public Education Study of the chemical-physical properties of gangliosides and their metabolism in cell in cultures

Study of the role of glycosphingolipids and their metabolism in plasma membranesTECHNICAL SKILLS AND COMPETENCESHer technical skills concern the analysis of lipid and protein composition of membranes and subcellular fractions from both tissue and cultured cells; study of metabolism and enzymology of gangliosides and sphingolipids, in particular distribution of their different molecular species; the development of stably transfected cell lines for both silencing and… Show more Study of the role of glycosphingolipids and their metabolism in plasma membranesTECHNICAL SKILLS AND COMPETENCESHer technical skills concern the analysis of lipid and protein composition of membranes and subcellular fractions from both tissue and cultured cells; study of metabolism and enzymology of gangliosides and sphingolipids, in particular distribution of their different molecular species; the development of stably transfected cell lines for both silencing and overexpression studies. The experimental competences include: lipid extractions; lipid chromatography; metabolic labeling of cells in culture with radioactive and/or photoreactive molecules; subcellular fractionation techniques; protein analysis by SDS-PAGE; cloning of sh-RNA and cDNA in eukaryotic expression vectors; stable transfection of mammalian cell lines fluorometric techniques; HPLC techniques, mass spectrometry techniques. Show less