The Master thesis 1-year internship was done at the genetics laboratory of the biotechnology and biosciences department of Milano-Bicocca University, with the aim to characterize the response to DNA damage in yeast, with molecular and computational biology methods. I have worked on RAP1, a yeast protein that has different roles, from telomere regulation to DNA damage response.• Research project focused on the study of the role of the Rap1 protein. Research and characterization of… Show more The Master thesis 1-year internship was done at the genetics laboratory of the biotechnology and biosciences department of Milano-Bicocca University, with the aim to characterize the response to DNA damage in yeast, with molecular and computational biology methods. I have worked on RAP1, a yeast protein that has different roles, from telomere regulation to DNA damage response.• Research project focused on the study of the role of the Rap1 protein. Research and characterization of allelic variants in order to understand the involvement of Rap1 in the response to DNA damage;• Use of the Saccharomyces cerevisiae model system;• Use and in-depth study of classical genetic techniques, molecular genetic techniques and molecular biology techniques;• Use of computational techniques for protein-protein and protein-DNA interaction study. Usage of Molecular modelling software and molecular dynamics methods. Show less

The internship project aimed to study some aspects of the conjunctival immune system in order to understand whether the differences in susceptibility to ocular infection between different murine strains, described by many papers, may be due to a different microbiota/IgA ratio as in the intestine.• Characterization of some aspects of the mucosal immune system of the murine conjunctiva, comparing animals with different genetic backgrounds. Retrotrascription extraction and quantification… Show more The internship project aimed to study some aspects of the conjunctival immune system in order to understand whether the differences in susceptibility to ocular infection between different murine strains, described by many papers, may be due to a different microbiota/IgA ratio as in the intestine.• Characterization of some aspects of the mucosal immune system of the murine conjunctiva, comparing animals with different genetic backgrounds. Retrotrascription extraction and quantification of mRNAs produced at the level of the conjunctiva.• Comparative analysis of the expression of specific mRNAs in different genetic backgrounds;• ELISA test to determine the concentration of total IgA, circulating in serum and mucosal, in tears and faeces • Cytofluorimetric analysis of cell extracts from the eye and sub-mandibular lymph nodes.• Use of immunology, cellular and molecular biology techniques as well as computational analysis. Application of PCR, RT-PCR, Real- time PCR electrophoresis, flow cytofluorimetry, ELISA, DNA / RNA extraction from cellular tissues, immunohistochemistry, cell cultures;• Participation in the journal clubs and lab meetings of the laboratory. Show less