Mantenimento e manipolazione di diverse colture cellulari neurali, sia primarie di origine murina (cellule staminali neurali, precursori dell'oligodendrocita) che linee cellulari immortalizzate umane e murine (SH-SY5Y, BV2).Estrazione acidi nucleici (DNA, RNA) da cellule o tessuto tramite estrazione fenolo-cloroformio e colonnine con membrana di silice. Elettroforesi su gel di agarosio.Retrotrascrizione, analisi dell'espressione genica tramite Real Time qPCR.Estrazione di proteine da cellule e/o tessuto, quantificazione tramite acido bicinconinico (BCA), analisi proteomiche tramite ELISA e Western Blot.

Standard laboratory procedures.Maintenance and manipulation of human and murine primary cell cultures (human brain microvascular endothelial cells, lung endothelial cells, pericytes, neural stem cells, oligodendrocyte precursor cells, cortical neurons and astrocytes).Cell Biology Techniques:- freezing/thawing cells;- cell isolation/propagation/differentiation/splitting;- cell counting by Burker chamber and automated cytometer;- cell treatment;- MTT assay.Direct and indirect immunofluorescence.Gene expression analysis.Molecular Biology techniques:- DNA and RNA extraction;- DNA and RNA quantification by NanoDrop spectrophotometer;- RT-PCR;- Real Time qPCR;- primer design and drawing.Microscopy and image analysis:- light microscopy;- fluorescence microscopy;- cell-based High Content Screening (HCS);- NIS-Elements;- ImageJ (morphological analysis and Sholl Analysis).Statistical programs:- GraphPad Prism.