Marcus Norris Email & Phone Number

Durham, Nc, Us

Durham, Nc, Us

● Participated in collaborations with external partners to facilitate the tech transfer of assays and culture methods for ongoing armored CAR and SEAKER CARCIK studies.● Maintained laboratory notebooks in compliance with regulatory standards, ensuring accurate documentation for ongoing research projects.● Performed monthly maintenance, calibration, and cleaning on flow cytometers, microscopes, incubators and other laboratory equipment and systems.● Kept inventories of laboratory reagents and supplies according to current project needs.(see also role as Scientist I at CoImmune)

● Collaborated on the development of cell culture methods and functional testing of CD19-targeted CAR cytokine induced killer (CARCIK-CD19) cells. ● Developed electroporation methods to maximize the transfection efficiency of T cells using a CD19CAR plasmid with Sleeping Beauty transposase. This work resulted in increasing the CAR receptor expression by up to 80%.● Developed and performed immunoassays to stimulate armored CAR T cells and measure cytokine release using ELISA and multiplex cytokine bead array (CBA) methods.● Coordinated with external partnerships to establish precise technical goals, driving the achievement of project objectives in assay development and technology transfer, method troubleshooting, communication with partners, and effective data presentation in support of the CARCIK-CD19 project.● Trained new hires on cell based immunoassays, flow cytometry and culture methods to support integration into Process Development group. Trained manufacturing personnel in CAR T cell transfection methods.● Participated in weekly interdepartmental meetings to present data, address challenges, and propose corrective adjustments to the CARCIK-CD19 and other projects.● Wrote tech reports, SOPs, and test method reports to summarize process improvements.● Performed technical review of data, summarized findings, prepared figures, and authored documents for publication.

● Led the tech transfer of Argos dendritic cell culture and bioassay methods to two international companies, CellThera, and Lummy, which helped bring in over 2 million dollars for Argos Therapeutics.● Designed and performed immunomonitoring assays to process over 200 patient samples and analyzed data in support of Argos clinical trials. ● Conducted research studies and developed immunoassays to identify the binding partners of sCD83, employing both cell-free binding assays and far-western blot techniques.● Performed cell-based assays to determine impact of chemotherapy drugs, and checkpoint inhibitors on Arcelis dendritic cell function and viability for feasibility study.● Chemical Safety Officer (2017-2018), responsible for maintaining material safety data sheets for current reagents and ensuring appropriate safe use and storage of materials.● Developed and performed protein-ligand binding experiments to visualize CD83 interactions with targets using fluorescence microscopy.● Authored tech reports and SOPs to summarize Research Immunology data and methods.

● Led the tech transfer of dendritic cell culture process improvements to manufacturing and efficacy assays to QC.● Performed flow-based phenotyping assays on dendritic cells during development runs to evaluate process improvements.● Designed protocols for the ELUTRA cell separation system to increase purity and yield of monocytes from apheresis products, and prepared recovery protocols to be used by manufacturing to prevent loss of patient material. ● Developed and performed ELISA assays to measure IL-12p70 produced by Argos' autologous dendritic cell immunotherapy.

● Led the tech transfer of finalized AGS-003 processes from development to manufacturing to complete key company goal.● Designed and performed immunphenotyping and ELISA assays to access DC function and efficacy during R&D and manufacturing runs.

Ms, Biology

Bachelor Of Science - Bs, Biology, General