Laboratory Manager of Phase II clinical trial on anticoagulation in APS; Investigating the mechanisms through which autoantibody populations cause thrombosis and pregnancy morbidity in antiphospholipid syndrome and systemic lupus erythematosus patients; standarisation and optimisation of laboratory testing for APS; development of specialised haemostatic assays.

Named Post doctoral in Phase II/III Clinical Trial : A prospective randomized controlled phase II/III clinical trial of rivaroxaban versus warfarin in patients with thrombotic antiphospholipid syndrome (APS) with/without SLE. Duties: Optimisation and standarization of all assays in the protocol according to clinical trial standards and regulations/Established reference ranges for each assay performedDeveloped methodology and established the lower limits of detection and sensitivity limits of all laboratory assays performed/Analysis of all data interpretation and preparation of reports and papers/Development of new equations for presentation of the results and troubleshootingDaily liaison with trial managers, member of the trial committee, attending meetings and updating chief investigators, clinical trial managers, co-investigators of results and progress/preparing reports for the steering committee, liaising with co-investigators at second site for samples and possible issues, attending regular meeting with trial managers/responsible for all paperwork in terms of blood sample collection, record keeping, results, the clinical trial files and aiding in final results analysis and interpretation. Additional projects involvement in anticoagulation in Antiphospholipids Syndrome, Pregnancy complications, Lupus anticoagulant, Intensity of anti-coagulation, Rivaroxaban in Antiphospholipid syndrome (RAPS) clinical Trial, APS action, ELISAS.Helped establish and I am actively participating in studies with strong collaborations with prominent rheumatologists, haematologists, and reproductive specialists . Actively recruiting and consenting patients by attending various clinics at UCLH: neonatal, reproductive medicine, haematology and Rheumatology, collecting and processing patient samples and preparing serum, platelet poor plasma, EDTA plasma.

Activated Protein C (APC) in Severe Sepsis: Generation and characterisation of novel APC variantsExtensive molecular, cell and biochemical laboratory work evaluation of secretion and electrophoretic migration patternsEstablish activation, relative anticoagulant activity in plasma-based assays, and cytoprotective activity in endothelial cell-based assays. Generated Murine APC variants for in vivo evaluation in murine models of stroke.Techniques and Experience: Gene amplification and cloning, RNA and DNA extraction, protein expression in mammalian cell systems, protein purification (FPLC), membrane blotting, ELISA, human and murine endothelial cell culture and maintenance, plasma-based (calibrated automated thrombinography) and purified assays, endothelial-based assays. TeachingPresentationsAttending of international and national meetings

Gene expression studies during embryonic developmentCollection and preparation of all human and murine embryonic tissues perform in situ hybridisation analysis of gene expression using human and mouse embryonic material, presentation of findings in group and departmental meetings analysis and preparation of reports.

Investigation of upregulation of genes during diabetic nephropathy processes in both rat and human. Identification of the candidate genes with different methods such as differential displayCloningdesign of primers transformationamplification with PCRstudy with bioinformatics methods extraction of RNA and genomic DNAcell culturequantitative real time PCR (light cycler)

‘Identification and classification of enterovirus based on the 5’ non coding region at sewage samples. PCRRT-PCRRFLP cell culturesRNA and DNA extractionResearch based on the coxcakie A virus.