Mariana Rocha Email & Phone Number

Vancouver, British Columbia, Canada

Multispecific Antibody Therapeutics - Discovery BiotherapeuticsRecombinant protein production and purification for in vitro or in vivo studies.Biophysical and biochemical methods for protein analysis.Cell Binding assays for screening of antibody variants.Experience with CROs for custom protein production.

Vancouver, British Columbia, Canada

September 2022 - February 2023 ○ Multispecific Antibody Therapeutics - Protein Engineering April 2022 to September 2022 ○ ADC (Antibody-Drug Conjugate) Therapeutic Development - Analytics- Antibodies Purification and Biophysical Characterization• Responsible for the discovery, generation and early development of therapeutics, as well as the development of innovative technology to create best-in-class biologics.

Vancouver, British Columbia, Canada

Protein Engineering - Responsible for the purification and characterization of novel antibodies therapeutics from discovery through to lead candidate selection. - Protein chromatography techniques including proteinA, IMAC, SEC, ion exchange, desalting and buffer exchange. Using AKTA chromatography system.- Detailed protein characterization using the following techniques: analytical chromatography (HPLC/UPLC), capillary electrophoresis (CE-SDS), light scattering (DLS, SEC-MALS), thermal stability assessment (DSF, DSC), binding affinity (SPR, KinExA) and developability assays (AC-SINS, NS-ELISA, cIEF).“Creating disruptive therapeutic platforms and impactful biotherapeutics that return patients to their loved ones, disease-free.”

Burnaby, British Columbia, Canada

Experience within an industrial cGMP environment.- Quality control testing on all finished and semi-finished products as well as raw materials.- Analysis of small molecules by HPLC- Participated in product development formulation- Planned and coordinated laboratory activities, documenting any information and findings in daily logs and lab notebooks.- Preparation of specific documentation, such as Certificate of Analysis, product statements and SOPs.- General laboratory and equipment maintenance and monitoring.

Burnaby, British Columbia, Canada

• Pharmaceutical manufacturing company:| Organizational and Documentation management skills - Preparation of reports according to the company's standards, follow current good manufacturing practices (GMP) and adherence to quality control rules established at the company.| Ability to establish effective work relationships and participate in a team culture. | Excellent attention to detail - Executed the analysis of the final product and quality focus.| Team-work and Communication skills - communication with people across multiple divisions, including working in collaboration with the QC department.| Training of new employees.

Ribeirão Preto Area, Brazil

• Laboratory of Microbiology and Cell Biology.| Experimental planning of a scientific project conducting screening, protein characterization and protein purification assays. Resulting in analytical method development for target enzymatic reaction.| Strong Biochemistry and Analytical Chemistry skills with experience in various assay techniques such as HPLC, enzyme activity assays, protein assays.| Collaborative team-based research. | Strong communication skills, developed by writing, arranging and correcting research data.| Improvement of a set of laboratory (biochemistry and molecular biology) technical skills. Responsible for a scientific project approved by FAPESP. It was performed under the supervision of Profa. Dr. Maria de Lourdes T.M. Polizeli.Dissertation: "Study of fungal enzymatic efficiency in the reactions of transesterification and hydrolysis from native vegetable oils targeting biotechnological applications."MSc degree in Biochemistry at Ribeirão Preto Medical School (FMRP-USP) - University of Sao Paulo.

Uberlândia Area, Brazil

• Laboratory of Virology| Enhancement of a set of laboratory (biochemistry, cell culture and molecular biology) technical skills. | Time management, Teamwork & collaboration, Communication and Organizational skills.| Conducted experiments to promote the expression and purification of recombinant antigens for the development of a potential vaccine against Hepatitis C disease, caused by the Hepatitis C virus (HCV), with the supervision of Prof. Dr. Jonny Yokosawa at Institute of Biomedical Sciences.Work of Bachelor degree Conclusion: "Use of hydrophobic interaction chromatography in the purification of a recombinant protein containing hepatitis C virus E1 and E2 protein segments."

Guelph, Ontario, Canada

• Actin Laboratory | Yeast culture cell lines maintenance, performed validation for protein purification method for a recombinant protein, assuring the reliability and accuracy of the scientific results. | Biochemistry and cell biology technical assays skills.| Team collaboration by making literature reviews to assess the potential impact on studies and improving on-going experiments.Project: "Co-polymerization reaction using yeast actin purified with Gelsolin 4-6."Supervisor: Prof. Dr. John Dawson

Uberlândia Area, Brazil

• Laboratory of Biochemistry and Molecular Biology.| Learned about scientific methodology, monitoring of laboratory activities, conducting experiments, promoting the assurance of quality control.| Development of laboratory skills.Scientific Initiation Project: "Evaluation of sodium nitrate supplementation in the redox balance in an experimental obesity model". Supervisor: Profa. Dr. Françoise Vasconcelos Botelho.

Uberlândia Area, Brazil

• Laboratory of Protein BiochemistryFirst contact with a laboratory environment, learned about equipment cleaning, biosafety, laboratory management, organization of samples and results. Practice the most commonly used laboratory techniques such as electrophoresis and solution preparation. Supervisor: Profa. Dr. Renata Santos Rodrigues.

Master'S Degree, Biochemistry

University of Sao Paulo/Brazil (USP) Laboratory of Microbiology and Cell Biology. Ribeirao Preto Medical School (FMRP / USP) - Department.

Bachelor'S Degree, Biotechnology

Federal University of Uberlandia/Brazil (UFU)

Bachelor’S Degree, Biotechnology

Exchange Program (B.Sc) - Biochemistry Graduation Extension Program - Science Without Borders Program.