Mariana Kirst

Mariana Kirst Email and Phone Number

Molecular Biology | Next-generation Sequencing | Method Development | Project Management @ SolusCell
Mariana Kirst's Location
Gainesville, Florida, United States, United States
About Mariana Kirst

Self-motivated and experienced scientist with combined experience in cellular and molecular biology, genomics, microbiome and next-generation sequencing data analysis. * Experienced scientist in analytical method development, process development/release/stability testing and management of technology transfer to quality control and across sites, * Outstanding teamwork skills and experience in managing research and clinical professionals on collaborative projects resulting in publications and grant funding.* Forward-thinking with an analytical mind-set and ability to identify emerging trends and technologies exemplified by involvement in cutting edge projects in gene therapy, induced pluripotent stem cells, CRISPR and genomics/bioinformatics.

Mariana Kirst's Current Company Details
SolusCell

Soluscell

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Molecular Biology | Next-generation Sequencing | Method Development | Project Management
Mariana Kirst Work Experience Details
  • Soluscell
    Director Of Operations
    Soluscell Feb 2024 - Present
    - Coordinate the development of single cell and single nuclei RNA isolation methods for NGS sequencing from several sample tissues and species (plant, animal and human) - Project management, implement processes for client onboarding and project workflow
  • Vvs At Thermofisher Scientific
    Lead Scientist, Assay Development Analytics
    Vvs At Thermofisher Scientific Sep 2020 - Oct 2023
    Alachua County, Florida, United States
    • Subject Matter Expert for ddPCR-based assays for strength and impurities; Purity by SDS-PAGE and Identity and Impurities by Western Blot (lead investigations, method development, customization, and implementation; analyze and summarize data); train junior analysts, write/review documentation.• Manage the pipeline of analytical methods for onboarding clients within VVS-ALA site and coordinate with the SMEs responsible for the implementation of these methods. • Attend client meetings to present method implementation timelines and discuss prioritizations, as well as method transfer from development to qualification. • Evaluate resources (people and materials) to schedule operations and ensure all work is performed within the expected timeline.• Evaluate Project scope for analytical methods development, testing and stability studies. • Method technology transfer assessment and execution
  • University Of Florida
    Research Assistant Professor
    University Of Florida 2014 - Sep 2020
    Research* Coordinated genome wide association project of PiZ patients, leading to the discovery of genetic variant associated with misfolded protein aggresome and fibrosis.* Developed and optimized allelic discrimination qPCR assay for genetic variant diagnostic.* Effectively managed and provided support in the setup, sequencing and bioinformatic analysis of whole exome, transcriptome (RNAseq), ATAC and microbiome next-generation sequencing libraries.* Designed and accomplished transfection, siRNA and rAAV infection experiments in cell culture and mouse models. * Successfully constructed and screened CRISPR sgRNA plasmids leading to the development of stable knockout serpina1 Huh 7.5 cell line.Teaching* Pulmonary Fellows Research Lecture: Genetic variation and microbiome studies in pulmonary diseases * Graduate level course lectures: Introduction to microbiome (theory and analysis)
  • University Of Florida
    Postdoctoral Research Scientist
    University Of Florida 2010 - 2014
    Research* Developed and implemented new innovative approach for detection of HCV and HBV drug resistance based on targeted next-generation sequencing.* Designed and optimized sequencing strategy for microbiome studies in chronic periodontitis and cystic fibrosis.* Effectively managed laboratory daily activities by supervising technical staff, maintaining SOPs, EH&S compliance and interacting with sales personnel.
  • University Of Florida
    Postdoctoral Researcher
    University Of Florida 2007 - 2010

Mariana Kirst Education Details

Frequently Asked Questions about Mariana Kirst

What company does Mariana Kirst work for?

Mariana Kirst works for Soluscell

What is Mariana Kirst's role at the current company?

Mariana Kirst's current role is Molecular Biology | Next-generation Sequencing | Method Development | Project Management.

What schools did Mariana Kirst attend?

Mariana Kirst attended North Carolina State University, Universidade De Brasília, Universidade De Brasília.

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