Mariana Kirst Email and Phone Number
Self-motivated and experienced scientist with combined experience in cellular and molecular biology, genomics, microbiome and next-generation sequencing data analysis. * Experienced scientist in analytical method development, process development/release/stability testing and management of technology transfer to quality control and across sites, * Outstanding teamwork skills and experience in managing research and clinical professionals on collaborative projects resulting in publications and grant funding.* Forward-thinking with an analytical mind-set and ability to identify emerging trends and technologies exemplified by involvement in cutting edge projects in gene therapy, induced pluripotent stem cells, CRISPR and genomics/bioinformatics.
Soluscell
View-
Director Of OperationsSoluscell Feb 2024 - Present- Coordinate the development of single cell and single nuclei RNA isolation methods for NGS sequencing from several sample tissues and species (plant, animal and human) - Project management, implement processes for client onboarding and project workflow -
Lead Scientist, Assay Development AnalyticsVvs At Thermofisher Scientific Sep 2020 - Oct 2023Alachua County, Florida, United States• Subject Matter Expert for ddPCR-based assays for strength and impurities; Purity by SDS-PAGE and Identity and Impurities by Western Blot (lead investigations, method development, customization, and implementation; analyze and summarize data); train junior analysts, write/review documentation.• Manage the pipeline of analytical methods for onboarding clients within VVS-ALA site and coordinate with the SMEs responsible for the implementation of these methods. • Attend client meetings to present method implementation timelines and discuss prioritizations, as well as method transfer from development to qualification. • Evaluate resources (people and materials) to schedule operations and ensure all work is performed within the expected timeline.• Evaluate Project scope for analytical methods development, testing and stability studies. • Method technology transfer assessment and execution
-
Research Assistant ProfessorUniversity Of Florida 2014 - Sep 2020Research* Coordinated genome wide association project of PiZ patients, leading to the discovery of genetic variant associated with misfolded protein aggresome and fibrosis.* Developed and optimized allelic discrimination qPCR assay for genetic variant diagnostic.* Effectively managed and provided support in the setup, sequencing and bioinformatic analysis of whole exome, transcriptome (RNAseq), ATAC and microbiome next-generation sequencing libraries.* Designed and accomplished transfection, siRNA and rAAV infection experiments in cell culture and mouse models. * Successfully constructed and screened CRISPR sgRNA plasmids leading to the development of stable knockout serpina1 Huh 7.5 cell line.Teaching* Pulmonary Fellows Research Lecture: Genetic variation and microbiome studies in pulmonary diseases * Graduate level course lectures: Introduction to microbiome (theory and analysis) -
Postdoctoral Research ScientistUniversity Of Florida 2010 - 2014Research* Developed and implemented new innovative approach for detection of HCV and HBV drug resistance based on targeted next-generation sequencing.* Designed and optimized sequencing strategy for microbiome studies in chronic periodontitis and cystic fibrosis.* Effectively managed laboratory daily activities by supervising technical staff, maintaining SOPs, EH&S compliance and interacting with sales personnel. -
Postdoctoral ResearcherUniversity Of Florida 2007 - 2010
Mariana Kirst Education Details
-
Botany/Plant Biology -
Biology/Biological Sciences, General -
Biochemistry And Molecular Biology
Frequently Asked Questions about Mariana Kirst
What company does Mariana Kirst work for?
Mariana Kirst works for Soluscell
What is Mariana Kirst's role at the current company?
Mariana Kirst's current role is Molecular Biology | Next-generation Sequencing | Method Development | Project Management.
What schools did Mariana Kirst attend?
Mariana Kirst attended North Carolina State University, Universidade De Brasília, Universidade De Brasília.
Not the Mariana Kirst you were looking for?
-
Mariana Kirst
Gainesville, Fl -
1gafisa.com.br
-
Mariana Kirst
São Paulo, Brazil
Free Chrome Extension
Find emails, phones & company data instantly
Aero Online
Your AI prospecting assistant
Select data to include:
0 records × $0.02 per record
Download 750 million emails and 100 million phone numbers
Access emails and phone numbers of over 750 million business users. Instantly download verified profiles using 20+ filters, including location, job title, company, function, and industry.
Start your free trial