Marie Leger Email & Phone Number

Saclay, Île-De-France, France

Bringing extensive expertise to the role of Research Consultant at Institut de Recherche Servier, I specialize in the dynamic fields of protocol development, optimization, and integral support for protein production. My proficiency encompasses antibody discovery and engineering, coupled with adept management of cell culture and cell banking processes. With a meticulous approach to my work, I ensure the precise maintenance of electronic laboratory notebooks, contributing significantly to the… Show more Bringing extensive expertise to the role of Research Consultant at Institut de Recherche Servier, I specialize in the dynamic fields of protocol development, optimization, and integral support for protein production. My proficiency encompasses antibody discovery and engineering, coupled with adept management of cell culture and cell banking processes. With a meticulous approach to my work, I ensure the precise maintenance of electronic laboratory notebooks, contributing significantly to the creation of a seamless and efficient research environment.In my capacity as a Protein Production Trainer, I actively share my knowledge, fostering the professional growth and proficiency of fellow team members. Show less

Fort-De-France, French-Martinique

As a committed Biomedical Lab Technician at Biosanté SELAS, I played a pivotal role in the Hematology, Biochemistry, and Immunology Departments, contributing to the efficient and precise functioning of laboratory operations. My responsibilities encompassed the comprehensive preparation of reports, lab management duties, and the drafting of experimental protocols. Proficient in the operation of advanced analyzers such as ADVIA (Siemens) and Octa (Sebia), IH500 (Biorad) I ensured meticulous… Show more As a committed Biomedical Lab Technician at Biosanté SELAS, I played a pivotal role in the Hematology, Biochemistry, and Immunology Departments, contributing to the efficient and precise functioning of laboratory operations. My responsibilities encompassed the comprehensive preparation of reports, lab management duties, and the drafting of experimental protocols. Proficient in the operation of advanced analyzers such as ADVIA (Siemens) and Octa (Sebia), IH500 (Biorad) I ensured meticulous preparation of analyzers, reagents, and biological samples. Show less

Région De Ottawa, Canada

As an Engineer's Assistant at the SPROTT CENTER FOR STEM CELL RESEARCH, I played a crucial role in advancing our understanding of the "Role of TLX 1 in T cell Acute Lymphoblastic Leukemia." This groundbreaking research involved a comprehensive range of responsibilities, including mice genotyping, cell line maintenance, construction of 3C libraries, xenograft procedures in mice, exploration of 3D genome organization, and the specialized focus on the formation of chromatin loops.Key… Show more As an Engineer's Assistant at the SPROTT CENTER FOR STEM CELL RESEARCH, I played a crucial role in advancing our understanding of the "Role of TLX 1 in T cell Acute Lymphoblastic Leukemia." This groundbreaking research involved a comprehensive range of responsibilities, including mice genotyping, cell line maintenance, construction of 3C libraries, xenograft procedures in mice, exploration of 3D genome organization, and the specialized focus on the formation of chromatin loops.Key contributions- Conducted precise mice genotyping, contributing to the identification of genetic traits crucial for understanding TLX 1 in T cell Acute Lymphoblastic Leukemia.- Manage the maintenance of cell lines, demonstrating proficiency in cell culture techniques essential for ongoing experiments.- Spearheaded the construction of 3C libraries, vital for exploring chromatin structure and interactions, including the formation of loops.- Execute xenograft procedures in mice, enhancing our in vivo understanding of TLX 1's role in leukemia.- Investigate the 3D organization of the genome, with a particular emphasis on the formation of chromatin loops, contributing to a deeper comprehension of spatial chromatin interactions. Show less

Fontenay-Aux-Roses, Île-De-France, France

During my Master's degree training at UMR 1274 / CEA / INSERM, I played a pivotal role in advanced research. My focus included the subject "Transduction assays of CD34+ cord blood cells to study the role of an oncogenic fusion in childhood leukemic development." my responsibilities encompassed a range of tasks, including the production of lentiviruses.Key Contributions:- Conducted comprehensive cytometry by flow analysis, advancing our understanding of cellular characteristics… Show more During my Master's degree training at UMR 1274 / CEA / INSERM, I played a pivotal role in advanced research. My focus included the subject "Transduction assays of CD34+ cord blood cells to study the role of an oncogenic fusion in childhood leukemic development." my responsibilities encompassed a range of tasks, including the production of lentiviruses.Key Contributions:- Conducted comprehensive cytometry by flow analysis, advancing our understanding of cellular characteristics crucial for childhood leukemic development.- Utilized cloning techniques to study genetic stability and the impact of the identified oncogenic fusion in cloned cell populations.- Contributed to CD34+ cell transduction assays, offering insights into the specific role of the identified oncogenic fusion in leukemogenesis.- Executed xenograft procedures in mice, enriching our comprehension of the in vivo implications of the studied oncogenic fusion.- Meticulously maintained electronic lab notebooks, ensuring precise documentation and adherence to research standards.Played a key role in the design of informative posters, effectively communicating research findings to the scientific community. Show less

Lille

During my Bachelor's internship at the Institute of Cancer Research of Lille, INSERM UMR s 1172, I engaged in a compelling exploration of "Metabolic alterations in myeloid leukemia cells treated with FLT3 inhibitors," with a primary focus on the resistance of leukemic cells treated with inhibitors. This investigation involved in-depth analyses utilizing Western Blot, Seahorse technology, and proficiency in cell culture techniques.

Villeneuve D'Acq

Key Contributions:- Investigated the physical and morphological characteristics of Bacillus sp through experimental methodologies.- Examined the interactions between Bacillus sp and microbeads, providing insights into potential applications or environmental implications.- Utilized microscopic techniques to analyze and document the observed properties, contributing to a comprehensive understanding of the subject.

Région De Lille, France

As part of my BTS in Biotechnologies, I undertook an internship at the Pasteur Institute of LILLE, focusing on the detection, identification, and description of Blastocystis sp. This experience allowed me to delve into the field of molecular biology, developing skills in molecular detection techniques and data analysis.

Certificat D'Aptitude Professionnelle, Project Management, Certified

Les méthodologies de gestion de projets (Agile, Waterfall, Cycle V) Les bases de la communication stratégique Savoir Fédérer son équipe.

Complete, Non-Certifying Module, Project Management

Méthodologies de gestion de projet : Formations gratuites Gestion d'équipe Identification et gestion des risques Résoudre les problèmes.

Master 2 (M2), Cell/Cellular And Molecular Biology

Activities and Societies: Self-Defense - Course à pieds

Licence, Communication Cellulaire Et Physiologie, Admise

Communication cellulaire Physiologie Biologie cellulaire Biologie du développement Génétique

Dec, Biotechnologies, Admise