Head of Business Development at Exosome Diagnostics

Ion Torrent™ Product lines responsible for:The Ion Personal Genome Machine™ (PGM™)Ion OneTouch 2 System™Ion Certified Service Provider Program

Laboratory of Francis S. Collins, Director, National Institutes of HealthFunctional Studies*Carried out experiments on genes implicated in diabetes risk*Participated in brainstorming meetings with Principal Investigator, supervisors, and post docs*Trained lab personnel in techniques, procedures, and use of equipment/roboticsCloning*Familiar with all steps *Worked with a variety of plasmid vectors*Familiar/Proficient in:imaging and use of fluorescent tags such GFP, Bacterial transformation, sequencing of colonies, transfection, and various DNA extraction and purifying techniques*Genome browsers and molecular biology software*Involved in the development of a Novel MALDI-TOF Mass Spec method to detect:Allele Specific expression & representation*Served as authoritative consultant to researchers on technical aspects of automated genotypingPublications, Awards, and Presentation*Distinguished Performance Award for 2008, 2009*Attended and presented in bi-weekly lab meetings, NHGRI intramural retreat, and conferences involving other Principal Investigators from Michigan, USC, and Finland*Pathways Tuition Award*Up to date with current literature*Attended NIH campus talks, events, conferences, and courses*Rotated part time in NHGRI Technology Transfer office in 2012*Represented NHGRI at the 2012 International BIO convention in BostonSelected Publications:Heritable Individual Specific and Allele-Specific Chromatin Signatures in Humans. Science. Apr 9 2010-McDaniell R, {6 others}, Morken MA, Kucera KS, Battenhouse A, Keefe D, Collins FS, Willard HF, Lieb JD, Furey TS, Crawford GE, Iyer VR, Birney E. Effects of 34 Risk Loci for Type 2 Diabetes or Hyperglycemia on Lipoprotein Subclasses and Their Composition in 6,580 Non-diabetic Finnish Men. Diabetes, May 2011-Alena Stančáková, {4 others}, Mario A. Morken, Francis S. Collins, Anne U. Jackson, Michael L. Boehnke, Johanna Kuusisto, Mika Ala-Korpela, Markku Laakso27 current co-author publications

Responsibilities-Processed samples using different technologies for profit generating production process-Hybridization of samples using Affymetrix Gene Expression Arrays and 500K SNP arrays-Helped with Process Improvement, Training, and Alternative Platform Evaluation -Contributed to developing accurate and efficient QC methods-Implemented measures to better track samples, and track individual lot numbers of reagents being used-Trained new employees and temporary workers in all processes-Familiar with genotyping software-Created SOPs and directly worked with QA department for approval Platforms and Technologies-cDNA, IVT, SNP, and Microarray production laboratory-Responsible for large studies hybridizing thousands of samples on Affymetrix SNP & expression arrays-Isolated and extracted DNA from the sample tissue-Processed samples from isolation through hybridization on microarrays-Familiar with multiple DNA extraction techniques with and without robotics-Agilent aCGH and ABI SNPlex-Involved in converting proof of concept to a service offering for both technologiesMolecular Techniques -Normalization of DNA, DNA quantitation, restriction enzyme digestion, DNA ligation, PCR, DNA purification, Fragmentation, End-labeling, hybridization, staining and scanning microarrays, etc.-cDNA and IVT reactions and magnetic bead cleanup of these reactions -Detection of DNA copy number variations across the genome using Agilent aCGH-Analysis of DNA and detection of SNPs through capillary electrophoresis using the ABI 3730 DNA analyzer