Mario Skarica, Md Email & Phone Number

- Human nervous system evolution, development and aging - elucidating human specific traits, transcriptomic programs and biomarkers of pathogenesis of neurological and psychiatric disorders- Leading the development of assays and protocols for analysis of tissue, isolation of cells and cell nuclei from: preserved human brain, perfusion preserved pig brain and internal organs, and preparation of chromatin and RNA for next generation sequencing (RNA-Seq, Chip-Seq, ATAC-Seq, Hi-C)- Physician-Scientist with expertise in neurobiology and immunology (neurodevelopment, multiple sclerosis, autoimmunity and cancer), both in animal models and clinically oriented investigations.- Successfully lead demanding projects focusing on lentiviral VIP immunotherapy and novel therapeutic application of Lestaurtinib in animal model of multiple sclerosis (EAE) and investigating immune system - modifying effects of Tysabri (natalizumab) on RRMS patients under current therapeutic regimen.- Neuroimmunology expertise and extensive experience in additional autoimmunity and pharmacological and cell therapy animal models, covering collagen induced arthritis, graft versus host disease, and tumor immunology.- Independently designed and lead experiments, interpreted results, with transfer of experimental and theoretical knowledge to many colleagues.- Exposed to and mastered numerous cellular and molecular biology techniques including gene therapy with lentiviral vectors, culture of cell lines and primary cells, flow cytometry, transfections, RNAi, DNA/RNA/RT-PCR, ELISA, Western blot, and various immunological functional assays

Listed skills include Flow Cytometry, Animal Models, Immunology, Rt Pcr, and 25 others.