Manage in vivo efforts and personnel in the Clodagh O’Shea and Alan Saghatelian labso Responsible for training new laboratory personnel in in vivo techniques and responsible experimental designo Oversee the introduction and ramifications of new animal models and coordinate with the Salk IACUC and laboratory personnel for protocol approval, execution, and complianceo Currently the lead pharmacologist for two small molecule drug programs performing proof of principal, early efficacy, and PK in vivo experimentsPerform a wide variety of in vivo techniques supporting studies in oncology, auto-immune disease, metabolism, wound healing, and others.

Senior member of the translation group in Clodagh O’Shea’s lab leading the in vivo efforts to develop oncolytic viral therapy to treat solid tumors as well as metastasiso Developed xenograft models for efficacy involving tumor measurements, luminescent and fluorescent imaging (IVIS), biodistribution, and histologic analysis o Performed toxicologic assessment of particle toxicity in mice, specifically hepatic toxicityand ascertain relevance to human

* Lead biologist working on exploiting PAMP sensing pathways of the innate immune system. Finishing proof of principal in vitro studies in cultured cells, starting primary cell assays and currently performing in vivo proof of principal studies as well as testing novel compounds. Additionally exploring the interconnection between innate and adaptive immune responses in relation to the tumor micro-environmento Set up an in vitro type 1 IFN assay to assess compound induction of IFNb thru PAMP sensing pathwayso Performed siRNA knockdown in THP-1 human monocytes to support method of actiono Evaluated gene product by qPCR and protein by westerno Differentiated THP-1 monocytes into macrophages, mouse bone marrow progenitor cells into mDC, pDC, and macrophages and differentiated hPBMCs into M1 and M2 macrophages to elucidate the method of action of our compoundso In vivo allograft models with B16F10 and CT26WT cell lines looking at tumor size reduction/reduced growth rate, IFNb induction by gene product slowing of contralateral tumor and reduction of metastasis in the lungs by both imaging and physical colony counts* Part of the team performing in vivo experiments in support of our proprietary GalNAc peptides for targeted knockdown of various proteins in the liver* Set-up and validated several in vitro assays including antibody dependent cellular cytotoxicity (ADCC), complement dependent cytotoxicity (CDC), antibody directed cellular phagocytosis (ADCP), C3a alternate pathway complement activation* Currently performing syngeneic allograft in vivo experiments* Responsible for the operation of the vivarium

* Lead a project to break B-cell tolerance for the production of antibodies to self-proteinso Ran this project independently from cloning, construct construction transient and stable transfection in mammalian cells, protein production, purification by gravity, analysis by western, BCA, and formulation to in vivo studies and endpoint analysis including FACS and ELISA. Project finished proof of principle studies and moved into hybridoma generation.* Part of a small team assessing two different technologies for an immunology project on Celiac diseaseo Established DTH in vivo studies using gliadin and OVA proteino Set up different adoptive transfer paradigms, prepared spleens into single cell suspensions, MACS purification, performed both H3 and Celltitre Fluor/Glo proliferation assayso Performed single and multiplexed ELISAs for cytokine induction/downregulationo Helped with extra-cellular and intra-cellular FACS staining and data analysis* Supported an immunology project in osteoarthritis by setting up both a DMM (destabilization of the medial meniscus) surgical model of osteoarthritis and an AIA model. Performed imaging using an IVIS imager to determine how well a bi-specific antibody targeted the affected region of the knee and assessed swelling to determine efficacy.* Supported a bi-specific antibody project by running CIA and CAIA in vivo models, ran ELISAs to assess cytokine regulation, and PK.* Setup the IACUC for animal study review and serve as the IACUC coordinator as well as chair* Collaborated with our veterinarian to write all of the SOPs for the vivarium* Performed xenograft and allograft studies in support of the Oncology department* Single handedly assessed the condition of the unused vivarium, detailed all facility concerns, received quotes for repairs, prepared and presented a comprehensive budget to modernize and repair the vivarium, received approval from the CEO and oversaw the project to completion

• Successfully conducted an antibody cloning strategy producing several novel IgG2a and IgG3 FC region mutations• Responsible for protocol design, amendment preparation, IACUC correspondence, conduct, data collection, and interpretation of all in-house Discovery Immunology In vivo studies in mice with engineered antibodies• Conducted both CIA (Collagen Induced Arthritis) and CAIA (Collagen Antibody Induced Arthritis) models of Rheumatoid Arthritis• Conducted anti-inflammatory PK/PD studies with therapeutic antibodies• Performed Ex vivo binding analysis• Designed and performed In vitro functional cellular assays with MULTIPLEX cytokine assay• Familiarity working in an AAALAC accredited vivarium

• Responsible for the coordination, conduct, interpretation, report writing, and database addition of all in-house Discovery Toxicology In vitro Essential Cell Function assays• Lead of micronucleus assay development• Responsible for protocol design, conduct, interpretation, and report writing of all in-house Discovery Toxicology In vivo studies in mouse and rat• Study Director of non-GLP In vivo toxicology studies• Supervised the setup of three DSE lab areas• Responsible for the coordination between Toxicology Department and CROs• GLP, OECD regulation competent• Maintain all study files for internal reference and work closely with document control• Review reports, prepare CTD tables, tabulate and analyze data of GLP and non-GLP studies.• Assist in writing summary reports and data presentation• Assist in the design, coordination, monitoring, and evaluation of non-GLP and GLP studies• Created and maintain study logs, electronic study logs, hERG data report logs• Review and summarize SBAs for pharmacology and toxicology review

* Lead of In vivo profiling group under Pharmacology* Lead of In vitro Screening department and proficient of all company wide assays* Developed several assays in the Screening department* Managed three Research Associates* Developed all SOPs for dissection, cryo-sectioning, immunohistochemistry, fluorescent microscopy for histological image analysis

* Purified mRNA and created full length dsRNA for gene silencing experiments (before the discovery of siRNA)* In charge of the radioactive area for the laboratory and trained colleagues how to perform Northern blots* Contributed to design, planning, and data evaluation of cellular and molecular experiments* Kept up to 12 cell lines in culture at any particular time to support the research efforts* Competent in a vast array of molecular biology techniques* Organized and participated in weekly team meetings