Mark Sever Email & Phone Number

Waterloo, ON, CA

Richmond Hill, ON, CA

Toronto, Ontario, Canada

• Lead human embryonic intermediate stem cell (hESCi) clinical batch production as process owner at both MaRS7 and CCRM cleanroom GMP sites, coordinating material transfer, line clearance and MP revisions/QA release
• Participate in clinical and development downstream drug substance production batches, which includes manipulating the AT- L1 vial filling machine
• Use Cell factories (CFs) of up to 40 layers as well as bioreactors (BioFlo320) for the scale up and propagation of iPSCs
• Author protocols, plan, coordinate, and execute operator aseptic qualifications (OAQs) and aseptic fill process qualifications (AFPQs), liaising with QC to coordinate testing events and QA for room release. Prepare.
• Use eQMS such as Veeva and MasterControl to write document change controls, SOP writing and revisions, coordinating with QA to ensure accuracy and regulatory compliance
• Procure equipment IQPQ as well as PM/equipment calibration, authoring qualification reports for GMP equipment

Mississauga, Ontario

-I am responsible for the propagation and scale-up of diverse mammalian cell-lines (Vero, Frhk-4, MA 104) for subsequent virus infection and harvest, following strict GMP and prescribed SOPs.-Produce sterile reagents intended for use in both cell-culture and processing, including buffers, growth mediums, and stabilizing chemicals used for harvest of.

Toronto, Ontario, Canada

-Investigated the protein-protein interaction of Actin and one of its regulators, Cofilin, to determine their role in the preservation of memory in the dendritic spines of neurons.-To that end, produced fluorophore-protein DNA plasmids using standard molecular sub-cloning techniques, which included the generation of bacterial cultures expressing these.

Toronto, Ontario, Canada

-Using the C. Elegans nematode as a biological model, investigated the effect of performing an RNAi- knockdown on an enzyme known to regulate the proper membrane-to-cytoplasm internalization of proteins required for proper development of the embryos.-Demonstrated problem-solving skills by setting-up appropriate conditions for crossing two different strains.

Toronto, Ontario, Canada

Project Title: Development and Optimization of the Core-Shell design and Inverse Core-Shell design of Quantum Dot Sensitized Solar Cells (QDSSC) -Demonstrated team work by collaborating alongside colleagues in optimizing the concentration of ionic species precursors in Chemical Bath Deposition (CBD) of CdS sensitizers onto TiO2 surfaces, including several.