Mark Sgro Email & Phone Number

Vancouver, British Columbia, Canada

Under the supervision of Dr. Poul SorensenUnraveling the surfaceome and global protein expression of metastatic osteosarcoma, with the aim of creating targeted immunotherapies.Technical work includes:• Hypoxic cell culture + Oxidative stress experiments• Spheroid generation and culture• Responsible for maintaining, expanding, and characterizing 10+ cell lines at a given time• Literature reviews, designing and executing experiments with PDX cell lines

Vancouver, British Columbia, Canada

Under the supervision of Dr. Megan Levings and Dr. Bruce VerchereDevelopment and validation of chimeric antigen receptor (CAR) regulatory immune cells for targeting stem cell-derived beta cells, with the aim of enhancing cell grafts for Type 1 Diabetes (T1D) therapy.Designed, executed, and validated a CRISPR Knock-in on a stem cell line. Confidently performed multi-colour flow cytometry (Fortessa, Symphony, Cytoflex) and Flowjo analysis, cell culture, ELISA, stem cell differentiation, and multiple cloning workflows (designing and building many CAR iterations). Other techniques and work involves:• RTqPCR• PBMC isolation • Immunomagnetic cell separation• Coaggregations of various cell types• Primary T cell differentiation and validation • Intracellular staining • STAT Phosphoflow• Legendplex (+ analysis)• Immunostaining and Confocal microscopy• FACS sorting • Cre-LoxP• Genotyping• Lentiviral Transduction• Lipofection• Designing and executing immunoassays• Data visualisation in PRISM/Graphpad• Culturing and preparation of cells for murine transplantations• GSIS• Preparation and proper handling of cytotoxic substances (STZ) for murine injections

Under the supervision of Dr. Joy RichmanHandled specimen processing, microtome sectioning, PCR, and histological staining (EdU, H&E). Demonstrated proficiency with software tools such as ImageJ and BLAST for data analysis. Engaged work with chicken embryos, in a developmental biology/dentistry-focused project.

Under the supervision of Dr. Sheila TevesResponsible for routinely characterizing and creating standardized stocks of analyzed plasmids. Technical skills include transformation/inoculation, working under flame, miniprep, restriction digest, and gel electrophoresis.

Under the supervision of Dr. Annie CierniaGained valuable research experience in brain tissue imaging and analysis in a lab studying microglia and the role of chromatin remodeling complexes in ASD. Technical skills include immunofluorescence, PCR, and brief work with neuron cultures.

Owen Sound, Ontario, Canada

Demonstrated expertise in visual merchandising consistently upholding stock maintenance standards in a retail setting, and assisted customers to provide tailored product recommendations and ensure a personalized shopping experience.

Owen Sound, Ontario, Canada

Proficiently operated and maintained a range of kitchen equipment at McDonald's, including grills and fryers, collaborating seamlessly with a dynamic team in a high-speed environment.

Bsc, Biochemistry

Notable courses: BIOC 304 Contemporary Biochemical Research BIOL 335 Molecular Genetics PCTH 325 Rational Basis of Drug Therapy PSYC 304.

Ontario Secondary School Diploma

Received the prestigious Governor General Award for academic excellence. Actively engaged in event planning for student-run clubs, notably.