Mark Bundy Email & Phone Number

Rockville, Maryland, Us

Rockville, Maryland, Us

• Develop robust, high-yielding, scalable, and economically viable cell culture processes to produce AAV products using miniature, bench scale and large-scale disposable. bioreactors Support development of upstream process development for viral vector and protein expression platforms.• Lead process scale-up and support GMP operations through technology transfer and process troubleshooting.• Collaborate and coordinate with relevant groups in technical operations and research and development.• Train junior team members on procedures and processes.

Waltham, Ma, Us

Research and Development - Scale Up - Protein Expression and Viral Vector Production (Upstream and Downstream)• Lead and develop a team of scientists in upstream and downstream process development.• Support development of upstream process development for viral vector and protein expression platforms.• Support development of downstream purification and characterization processes for viral vectors and protein expression platforms. • Provide process development support for all current and future transient transfection based products.• Provide support and troubleshooting for internal and external customers. Collaborate with cross functional teams across company divisions to align processes and support scale up projects to achieve company goals. • Author technical documents, customer reports, protocols and application notes.

Waltham, Ma, Us

Research and Development - Scale Up - Protein Expression and Viral Vector Production.• Lead scientist in charge of building a scale up program that develops scalable processes for all protein expression and viral vector production platforms in stirred tank bioreactors, rocking motion bioreactors, and microbioreactors from 15 mL to 50 L scale.• Provide customer support and troubleshooting for the upstream scale up of all transient transfection processes.• Develop and optimize scale up protocols for transient transfection of viral vectors (LV and AAV) in 293F suspension and SF9 suspension platforms.• Develop and optimize scale up protocols for transient transfection of protein expression for CHO, 293F and SF9 suspension platforms.• Collaborate with cross functional teams across company divisions to align processes and support scale up projects to achieve company goals.Author technical documents, customer reports, protocols and application notes.Tech transfer of scale up processes into CDMOs.Develop, analyze, and present DoE studies.

Basel, Ch

Research and Development, Media• Senior associate in support of protein production and media development for Chinese Hamster Ovary cell media and feeds. Utilized Design of Experiments (DOE) strategies to develop high-throughput screening experiments in Sartorius' AMBR15 to optimize media and processes, troubleshoot problems and screen components. Analyzed results through statistical software, including JMP and MODDE, and advised team on direction of the project. Used data analysis and literature to design experiments and find data driven solutions. • Technical lead in perfusion for 5L benchtop stirred tank reactors and the AMBR15.

Gaithersburg, Maryland, Us

Upstream Process Development - Scale Up Group• Lead researcher in the execution of high throughput process development studies using Sartorius AMBR15 micro bioreactor system. In charge of developing, executing, and analyzing Design of Experiments using JMP statistical software. Worked with interdisciplinary groups to analyze data. Advised group members on future directions of the project. • Primary associate in the process development of ATF perfusion in benchtop stirred-tank bioreactors to increase insect cell yield. • Responsible for coordinating with other teams to ensure their working virus stock needs were always fulfilled. Guaranteed on time delivery of working virus stock while being flexible with timelines and strains required.

Gaithersburg, Maryland, Us

Upstream Process Development - Cell Bank and Virus Stock Group• Supported manufacturing through confirmation of successful upstream process through the analysis of quality of infection and protein production. Prequalified all lots of media received from vendors to ensure product quality consistency and advised if each lot should be utilized in manufacturing. • Responsible for receiving and fulfilling cell stock orders from multiple groups across multiple departments. Guaranteed on time delivery of cells at the requested volume and cell density, and gave technical advice on the cell specific assays being performed. • Performed small scale long term media stability and process development studies in shake flasks. Analyzed and summarized results. Authored scientific reports and SOPs based upon the findings.

Cn

Research and Development, Vaccines• Responsible for assisting the primary investigator in the adaptation of seasonal influenza virus to both eggs and mammalian cells. Developed assays to investigate process parameters to increase viral yields. Isolated viral RNA and performed sequence analysis to identify mutations during adaptation. Ensured virus stocks were kept up to date and provided virus stocks to team members as they required. • Worked on stable cell line generation. Designed expression vectors, cloned genes of interest and transfected mammalian cells. • Assisted with 50L bioreactor experiments.

B.Sc, Biochemistry Cellular And Molecular Biology

Master Of Science (Msc), Biochemistry And Cellular And Molecular Biology