Marlene Pfeffer Email & Phone Number

State Of Goiás, Brazil

Genoa, Liguria, Italy

As part of an international European consortium focused on developing innovative treatments for blindness, I worked within a multidisciplinary, international group, contributing to groundbreaking research in neuroscience.- Managed and executed neuroscience research projects, playing a key role in the study of photosensitive molecules for vision restoration.- Independently developed, designed, and conducted experiments, managing data collection and dissemination of research findings.- Collaborated with multidisciplinary teams across an international consortium, establishing strategic partnerships with Key Opinion Leaders (KOLs), and ensuring the timely delivery of project outcomes.- Authored regular reports on work packages and goals as part of a European consortium, showcasing strong project coordination and documentation skills.- Presented research findings at 6 international conferences and published in high-impact journals, engaging healthcare professionals (HCPs) and contributing to the advancement of the scientific community.- Prepared primary hippocampal neuronal cultures and conducted comprehensive molecular characterization using advanced techniques.- Characterized light-sensitive molecules in neurons through whole-cell patch-clamp recordings, calcium imaging, and confocal microscopy.- Optimized molecular testing processes in neurons to enhance research efficiency and improve data quality.This work was conducted under the European project “enTRAIN VISION”, funded by the Marie Skłodowska-Curie Actions (MSCA) (Grant agreement No. 861423).Lighting up neurons: Characterization of New Membrane-Targeted Photoswitches for Geneless StimulationSupervisor: Prof. Fabio Benfenati

Bordeaux, Nouvelle-Aquitaine, France

“Dissection of Striatal Dopamine Hub Synapses in Physiology and Models of Addiction”Supervisor: Dr. Etienne Herzog- Experimental Design, Execution, and Data Analysis: Conducted preclinical research to understand the molecular mechanisms underlying Parkinson's disease. Developed and optimized experimental protocols to identify potential drug targets.- Project Management: Effectively managed laboratory operations, ensuring experiments were conducted efficiently and to the highest standards.- Training: I trained 3 new lab members fostering a collaborative and productive lab environment.- Scientific Communication: Presented research findings at a leading international conference and published original research as the first author in a high-impact journal.- Optimization of sorting of dopaminergic synaptosomes through Fluorescence-Activated Synaptosome Sorting (FASS) for proteomics assay- Characterization of protein profile of dopaminergic synapses through Western-blotIndependent development, design, and data collection and dissemination of results of experiments

Montreal, Quebec, Canada

“Early impairments of synaptic modulation at neuromuscular junctions in ALS SODG37R mousel model”Supervisor: Prof. Richard Robitaille- Contributed to a preclinical ALS study- Managed experimental protocols, data collection, and analysis- Dissection of soleus, mouse limbuscle- Characterization of electrophysiological properties of muscle fiber through Intracellular recordings- Characterization of muscle fiber type through Immunofluorescence and Confocal microscopy

Bordeaux, Nouvelle-Aquitaine, France

“Characterization of dopaminergic synapses using a cell sorter”Supervisor: Dr. Etienne Herzog- Sorting of dopaminergic synaptosomes through Fluorescence Activated Synaptosome Sorting (FASS)- Characterization of protein profile of dopaminergic synapses through Immunofluorescence- Analysis of precise proteinic localization through STED microscopy

Doctor Of Philosophy - Phd, Neuroscience, With Honors

Master Of Neuroscience

Bachelor Of Biotechnology