Mary Everett Email & Phone Number

• Fresh Blood and Leukopak separation and isolation of PBMCs using ficoll density gradient centrifugation• Post ficoll isolation of Neutrophils, pan DCs, CD4, CD8, CD56, CD14, CD20, T cells, B cells both manually and using StemCell Robosep• Receive, process and track research requests until completion• Prepare specimens and requisitions for shipment to laboratories worldwide• Perform established flow cytometry protocols to assess quality control of daily isolations and record… Show more • Fresh Blood and Leukopak separation and isolation of PBMCs using ficoll density gradient centrifugation• Post ficoll isolation of Neutrophils, pan DCs, CD4, CD8, CD56, CD14, CD20, T cells, B cells both manually and using StemCell Robosep• Receive, process and track research requests until completion• Prepare specimens and requisitions for shipment to laboratories worldwide• Perform established flow cytometry protocols to assess quality control of daily isolations and record results in the database• Identify unusual results/ outcomes and apply advanced troubleshooting techniques to resolve discrepancies• Provided input for streamlining processes within the research and development department and facilitated the communication and standardization of procedures in an effort to reduce errors and improve results of experiments• Train associates in protocols and operations of the blood core department• Present data and results regularly to researchers and upper management• Write and and review Standards of Procedure as well as spearhead development of current onboarding procedures • Equipment calibration and sterilization• Cell staining• Preparation and shipping of cryopreserved cells, • Regularly audits to ensure accuracy of data, • Maintain detailed laboratory notebooks and documentation Show less

• Cell Banking (master, archive, and working) • mycoplasma testing• CompacT testing• Preparation of FFPE and frozen Histogel pellets• Establish and capture model cell line characteristics and growth properties• Grow, split, passage and maintain contamination-free mammalian cell lines, both suspension and adherent• Manage and upkeep of cryopreserved specimens within the cryo laboratory• Extraction of DNA from mycoplasma sample• Development of protocols for… Show more • Cell Banking (master, archive, and working) • mycoplasma testing• CompacT testing• Preparation of FFPE and frozen Histogel pellets• Establish and capture model cell line characteristics and growth properties• Grow, split, passage and maintain contamination-free mammalian cell lines, both suspension and adherent• Manage and upkeep of cryopreserved specimens within the cryo laboratory• Extraction of DNA from mycoplasma sample• Development of protocols for cellular robotics• Ensure the accuracy of data entered in the Research Management system by performing regular audits and helping management to identify root causes of recurring errors Show less

• Formulated and implement well balanced lesson plans• Explained complex concepts using easy-to-understand terms• Interpersonal and communication skills• Interacted and motivated students in a positive manner to achieve academic goals, • Assisted and guided students with school and science fair projects• Worked one-on-one with students aged 7 to 18 with diverse learning needs and from diverse cultural backgrounds• Developed learning strategies and action plans to meet… Show more • Formulated and implement well balanced lesson plans• Explained complex concepts using easy-to-understand terms• Interpersonal and communication skills• Interacted and motivated students in a positive manner to achieve academic goals, • Assisted and guided students with school and science fair projects• Worked one-on-one with students aged 7 to 18 with diverse learning needs and from diverse cultural backgrounds• Developed learning strategies and action plans to meet student’s academic needs and increase comprehension and grades resulting in an average of 1.1 point increase in GPA• Prepared reports on student performances and concerns to communicate and update parents on student’s progress• Assessed barrier to learning on an individual basis, and tailored tutoring and teaching methods to each student, with a focus on optimizing engagement Show less

Bachelor Of Applied Science (B.A.Sc.), Biology/Biological Sciences, General

Activities and Societies: Tau Beta Sigma, Marching Band, Jazz Band

Master'S Degree, Biotechnology