Analysis:o Heterotrophic plate count in watero Total and Faecal coliform in watero E.coli in watero Total coliform and E.coli tests using colilerto Yeast and Mould in watero Pseudomonas test in watero Clostridia in watero Biological oxygen demand testo Enumeration of helminths Ova in sludge o LIMS labwareo Sulphur reducing bacteria testo Faecal coliform test in sludgeQuality assuranceo Method validation and repeatabilityo Reporting and checking of resultso Reviewing of resultso Liaising with clientso Quality control checks in the microbiology laboratoryo Improvement of existing methods and standard operating procedureso Problem solving in sectiono Overseeing sub ordinateso Internal auditor(in training)o Requesting for laboratory media chemicals and consumables to be ordered to ensure continuation of analyseso Training of staff in section on instrumentation, methods and standards operating procedureso Reviewing of documents and records of the management system and the initiating of changes to documents where relevant o Assist Control Microbiologist with report writing on a monthly basis o Ensure that the quality control functions including good laboratory practices are done in accordance to ISO 17025.o Approving results on the certificate of analysis – Technical Signatory for all accredited Microbiological methods

Performing gentamicin bio-assay on sponges impreginated with antibiotics and raw materials.Routine endotoxin testing on products, intermediates and water samples.Environmental monitoring of the clean room.Microbiological analyses of environmental monitoring samples.Calibration and maintenance of laboratory equipments.Water analysis on water for injection used during manufacturing.Preparation of media used for product analysis.Method validations on current sop method used.Accurate documentation and record keeping relating to the above analysis and testing.

Preparing media and reagentsChecking sterility of media by performing negative and positive controls Decontamination of used media plates and brothsCleaning validation of manufacturing machinesExtraction of plant materialsGrinding plantsPerforming minimum inhibitory concentration on plants used in tree projectCleaning the labInventory keeping

Identification of bacteria using gram staining and microscope Sub-culturing of bacteria.Analysis of raw materials, finished products and packaging materialsWriting reports for analysed raw materials, finished products and packaging materialsDaily calibration of weighing balance, and verification of the incubatorsAssisting with enviromental monitoring of the manufacturing areaAnalysis of water used in the manufacturing areaPreparing media and reagentsChecking sterility of media by performing negative and positive controls Decontamination of used media plates and brothsCleaning validation of manufacturing machines

Preparing practicals and assisting students at University of Limpopo during their practical sessions and also assisting with interpretation of their resultsIdentification of bacteria using gram staining and microscope Sub-culturing of bacteria.