Matthew Doyle Email and Phone Number

Key Skills • Lowry/ BCA/Park Johnson/AHMT• HPLC Subject Matter Expert• Empower (Empower 2-3)• HPLC troubleshooting certified• Good manufacturing practices (GMP)• Technologist Process Management • Equipment Subject Matter Expert• HPLC / GC maintenance certified

Testing and recording (GDP) the quality (GMP) of a wide variety of Merck Millpores medical and research devices using techniques such as:- Total Organic Carbon (TOC) detection (Membrane and Liquid samples) - Measuring viscosity using viscometry- High Performance Liquid Chromatography (HPLC) (Manual and Automatic Injection)- Empower- Scanning electron microscopy (SEM)- Optical densitometry (OD) - Western Blotting- Gas Chromatography- Fourier-transform infrared spectroscopy - Moisture meter - Cloudpoint- Pipette calibration- Water permeability (Flux)- Corr integrity

• Performing a wide variety of activities to support the introduction of new products to the site. • QC rep on site NPI teams and global analytical teams related to specific projects and products • Ensures lab readiness to perform raw material, in process and release testing of new clinical and commercial products. • Cordinates the transfer of analytical methods to the QC labs. • Performing data review and approval. • Providing support and advice to manufacturing on QC related topics. • Initiating and leading investigations where required. • Initiates and completes, CAPAs and Change Controls in accordance with site procedure • Writes new and updates current procedures on electronic documentation system. • Providing training to other analysts and to manufacturing personnel. • Presents technical analytical data clearly and concisely data to customers, i.e., internal investigations and regulatory inspectors etc. • Proactively identifies and implements lab process improvements. • Supports lean initiatives in the area of lab operations, i.e. standard work, level loading, • Supports Team leader on day to day testing operations.

• Maintenance of adherent and non-adherent human cell cultures• Maintenance of primary cell cultures (low and high volume)• Protein extraction• DNA extraction• Protein quantification by ELISA and Western Blotting• Plasmid propagation, transformation and transfection• In-solution, In-Gel and FASP digestion of protein samples for Mass Spectrometry • Q-ToF and Q-Exactive analysis of protein digests• Analysis of proteomic data files using Perseus, MaxQuant, MASCOT, PEAKs IPA and Cytoscape • RT-PCR• SNP analysis and identification• Immunoprecipitation• Molecular biology