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Biotechnology professional with over 8 years hands-on experience in a laboratory and FDA-regulated analytical and R&D environment. Experience includes strong oral and written communication skills of the presentation and documentation of scientific results in a fast-moving, integrative, and collaborative team environment;Method development design for quality control and analytical service assays; Automation experience in improving upon manual SOPs and test methods; Generation of AAV vectors in an investigational laboratory setting; Utilization of HPLC tools for the purification of viral vectors used in multi-disciplinary laboratories; Employment of root cause analysis approaches in order to improve AAV vector production and process; Critical analysis of generated analytical data sets to influence the investigational process; Extensive cell culture techniques in maintaining multiple cell lines; Balancing experiments involving viral vector production, transient transfection of mammalian cell lines, and quality control of generated viral vectors; Collecting and analyzing data for formal scientific publications; utilizing strong analytical skills to develop and follow new experimental protocols and SOPs; Maintaining and recording detailed laboratory documents; Producing and Maintaining stably transfected mammalian cell lines; confident Microsoft office and computing skills; excellent interpersonal and communication skills with various project managers.
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Associate ScientistCapsida Biotherapeutics Jul 2021 - Present- Qualify the qPCR assays used for Bio distribution studies to inform AAV capsid variants and their performance- Generate and track the performance of controls and standards used in qPCR and ddPCR assays-Assist other groups in processing and extracting nucleic acids from various AAV animal studies-Execute and run Bio distribution qPCR assays to inform various programs' animal studies-Maintain HEK293 cell lines used for AAV vector production-Assist other groups in the generation of AAV libraries to screen for novel capsids-Participate and consult on program teams-Train other technicians on executing our routine Bio distribution assays- Collaborate with other departments to improve and optimize our groups assay- Work with our internal automation team to bridge our manual qPCR method to a higher throughput process -
Associate ScientistKite Pharma Apr 2018 - Jul 2021Greater Los Angeles Area-Collaborate with other groups to develop and remediate assays used to test products with the goal of improving analyst time and to reduce assay invalid rates-Employ automation initiatives to help improve upon manual assay efficiencies such as DNA extraction-Drive lab operation projects to organize labspace and improve overall efficiency by using methods such as 5s-Improve group culture by keeping open lines of communication with all stakeholders and honestly addressing challenges as they arise-Utilize ddPCR and qPCR techniques to develop assays necessary for analytical development and quality control.-Maintain scientific records and notebook entries and analyze scientific data to be presented in a clear and consistent manner.-Utilize Flow Techniques to assist in the development of viral vector characterization and titers-Develop and use ELISA methods to asses vector safety and identity-Critically analyze generated data sets to influence the development of various QC and Analytical assays.-Employ good experimental design through DOE practice and JMP software to create robust test methods that are fit for purpose and follow ICH guidelines-Collaborate within and between departments to improve upon analytical assays utilized by them by provided data in a timely and organized manner. -
Senior Research AssociateAmgen Mar 2015 - Dec 2017Thousand Oaks, Ca- Demonstrate strong oral and written communication skills of the presentation and documentation of AAV production and DOE in an integrative, fast-moving, and collaborative team environment.- Experience in an investigational laboratory setting generating over eighty AAV vectors for other research groups and DOE experiments.- Purify and characterize viral vectors through the utilization of HPLC tools for use in multi-disciplinary laboratories as well as in the design of SOP optimization.- Work independently to employ strong root-cause analysis approaches in order to improve AAV vector production and process.- Critically analyze generated data sets to influence the investigation process testing various ligand densities to further improve AAV production and purification.- Demonstrate good understanding of biosafety practices while maintaining cell cultures, transfecting into mammalian cells, concentration of viral vectors, and performing quality control of viral vectors through titration and ELISA.- Maintain scientific records and notebook entries and analyze scientific data to be presented in a clear consistent manner.- Organize and consolidate back logs of over thirty different legacy cell lines -
Senior Research AssociateCytolumina Jan 2012 - Mar 2015Greater Los Angeles Area- Collect data from over seventy70+ clinical pancreatic cancer patients for scientific publication, which correlates circulating tumor cells (CTCs) and disease progression.- Employ various laboratory skills including blood lysis, immunohistochemistry, and CytoLumina’s proprietary CTC enumeration assays to process and analyze patient samples from various medical clinics including Cedars-Sinai and UCLA medical centers.- Write protocol for the utilization of CytoLumina’s novel nanotechnology: the CTC enumeration assay, which is in the application process for FDA’s 510k clearance. Design and employ various experiments for interferences testing, limit of detection (LoD), and reproducibility. - Train and manage multiple incoming technicians in utilizing the CTC enumeration assay and associated laboratory procedures.- Develop protocol for the culture and staining of the breast cancer cell line: SkBr-3, which is used to calibrate the CTC enumeration assay.- Coordinate many simultaneous projects, from patient sample analysis to the growth of one to two million SkBr-3 calibration cells. Work efficiently and independently while communicating effectively and professionally.
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PharmacologyUcla Jan 2012 - Jul 2012-Performed over seventy experiments with novel nanotechnology using clinical samples from pancreatic cancer patients.-Trained ten new technicians on laboratory procedures as well as proprietary nanotechnology techniques.
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Lab TechnicianUcla Ecology And Evolutionary Biology Apr 2011 - Aug 2011-Maintained over one hundred zebra fish stocks to be used in various genetic experiments-Collaborate with other technicians and researchers to effectively breed fish stocks.
Matthew F. Education Details
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Biotechnology -
Marine Biology And Biological Oceanography -
Biology, General
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UclaMarine Biology And Biological Oceanography
Frequently Asked Questions about Matthew F.
What company does Matthew F. work for?
Matthew F. works for Capsida Biotherapeutics
What is Matthew F.'s role at the current company?
Matthew F.'s current role is Associate Scientist.
What is Matthew F.'s email address?
Matthew F.'s email address is mf****@****gen.com
What is Matthew F.'s direct phone number?
Matthew F.'s direct phone number is +180544*****
What schools did Matthew F. attend?
Matthew F. attended University Of Maryland Global Campus, University Of California, Los Angeles, University Of California, Los Angeles, Ucla.
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