-Performed/supported preclinical activities for development of RSV and Influenza vaccine projects.-Performed characterization of monoclonal antibodies and polyclonal immune response by molecular, immunological and virological assays such as ELISA, HAI, neutralization assays.-Performed and optimized cell based functional assays for antibody in vitro functional characterization.-Worked with Cell based assays and mammalian cell culture.-Experience with monoclonal antibody technology.-Worked in a GLP, GLCP, GxP environment

-GLP, and PPE environment. -Method Development, Validation, and sample Analysis for pharmaceutical research.-Anti-drug antibody (ADA) testing for various sponsors.-Two types of assays and techniques used for screening immunogenicity assays, ELISA and Meso Scale Discovery (MSD).-Acid dissociation, Solid-phase extraction with acid dissociation (SPEAD), -Affinity purification or purification of total immunoglobulin from study samples prior to analysis, to improve sensitivity and drug tolerance of ELISA and MSD.-Bioassays with various endpoints, enzymatic assays, and ligand-based assays. -Class-specific affinity and Antigen-specific affinity for antigen purification.

-Quality control assays for cell therapy and cell based testing, Clonetics, Poetics release and stability test samples.-Performs a variety of analytical assays including FACS (Flow), cell-based potency, Sterility (BACTEC), Nucleocounter (Cell Count), ELISA and Elispot assays. -Writes, reviews and approves Test Records, Change Controls, Deviations and other Quality documentation required for cGMP sampling and testing, drafts, edits and revises SOPs-Participates in Batch Viral product testing (Wet Chemistry, HPLC, UV-Vis Spectrophotometry). -Sample and test raw materials for GMP compliance in an aseptic environment.▪ Growing, testing viability, cell staining, cells including HUVEC, and CD 34+

-Method Development and Validation for Bioanalytical Assays and Metabolite Profile studies. (UV Reproducibility, Extraction Efficiency, Protein Precipitation Extractions).-Transferred and developed analytical methods for extraction, HPLC fractionation and specialized Accelerator Mass Spectrometry analysis of samples.-Worked with elemental analyzer, Oxidizer, Liquid Scintillation Counter, and HPLC/uHPLC (Agilent 1200/1290) for pharmaceutical drug analysis. Used OpenLab software.-Ordering/purchasing. – developed a process for the correct distribution of purchase orders and packing slips from incoming consumables.-Conducted data processing, QC checking, and data compilation in preparation for report writing.-Contributed to the generation of study protocols and reports.-Prepared and updated SOPs and other generic documents (e.g. worksheets and spreadsheets) as required to contribute to a culture of continuous improvement.-Worked in accordance with the Company’s cGLP and cGCP quality procedure.

-Analysis of Chromatography and Mass Spectrometry peaks in correlation with forensic toxicology analytes. GCMS and LCMS.-Extraction and processing of urine, meconium, blood, and various serums using SOPs.-Finding pending specimens and routine maintenance on equipment.-Using Tandem, Documentum, Overlook, MultiQuant and SDS programs.-ELISA, HPLC, DSX, and Olympus.-Size-exclusion chromatography and ion exchange chromatography for protein purification.

-Performed and assisted with pH, EC, and scale-up tests, moisture content and soil particle analysis, Kcal inorganic extraction, soil fertility analysis, and Nitrogen Volatilization analysis.-Worked with fertilizer and nitropyridine to reduce nitrogen run off and atmospheric decomposition

Assisted with the Maryland Energy Administration – reviewed county policies and helped to write policies to reduce consumption of fuel in the Frederick county fleet and increase the county’s use of renewable energy. Determined energy baselines and tracking.Analyzed the flood zone/watershed area of the Potomac RiverAssisted with Green Homes Challenge and Solarize Frederick. Assisted participants with completing the Power Saver, Green Leader, and Renewable Star challenges, tracking and data collection via the web.Created a Storm Water Management Master Plan for Point of Rocks, Maryland and Maintained watershed riparian buffers and took off protective bird nets.Attended Accounting for Growth for Maryland meetings.