Matthew Adamow Email & Phone Number

New York, Ny, Us

Greater New York City Area

Multiparameter Flow Cytometry Assay Development, Validation, & Standardization* Created a 29-color T-cell Exhaustion/Activation Panel for the A5 symphony (x50)Multi-Institutional Collaboration with Upenn, UCSF, Stanford, & MD Anderson*Standardization of A5 Facs Symphony

Expertise Overview:Multiparameter Flow Cytometry Assay Development, Validation, & Standardization. * 16-color panel in-vitro stimulation assay * 11-color T-cell Effector/Exhaustion Panel * Myeloid Derived Suppressor Cell (MDSC) Panel * Flow Phosphorylation Assay: Procedure and staining panel development on whole blood and cryopreserved PBMC to measure pERK levels. Clinical Trial Monitoring: * Ex-vivo flow cytometry staining for T-cell effector/exhaustion panel * 10 day peptide stimulation for tetramer and polyfunctional cytokine staining * Tumor Infiltrating lymphocyte (TIL) flow staining. Data Analysis & Summarization * FlowJo Analysis and workflow development * Clustering and dimensionality reduction analysis (SPADE, t-SNE, PCA, etc) * Summary & presentation of trial monitoring development to PI’s and outside groups. Notable Collaborations with PI’s, Biotech companies, and other institutions. * UPenn: Pooled T-cell effector/exhaustion panel analysis of anti-PD-1 treated patients. * Serametrix: Developed an MDSC panel and validated stability of use of novel Cyto-chex BCT tubes to characterize myeloid derived suppressor cells. * CIC: Headed the IMFs participation in the Cancer Immunotherapy Consortium(CIC) whose aim is ICS gating standardization across multiple sites. * Pe’er Laboratory: Comparison of FlowJo analysis with various machine learning methods Overview of Equipment/Software Experience: * LSR Fortessa * Blood Processing and cryopreservation * Flow Cytometry Analysis Software: * FlowJo * Clustering analysis software such as SPADE * Dimensionality reduction analysis software (ie: Phenograph, PCA, t-SNE, viSNE, Wanderlust) * MATLAB, Prism * Enzyme-Linked ImmunoSorbent Assay (ELISA) * Meso Scale Discovery (MSD) Electrochemiluminescence assay

Memorial Sloan-Kettering Cancer Center

Our primary research aim is to develop and perform immune monitoring assays to quantify immunological responses in patients receiving novel cancer therapies. We are trying to understand and elucidate the mechanisms of antigen-specific T cell responses that arise from DNA vaccines and human monoclonal antibodies.

-Determining proteins and the biochemical pathway involved with RNA interference mechanism by using PCR, RT-PCR, Western Blotting, Dual Luciferase Assay, Silver Staining and Immunofluorescence -Elucidation and discovery of P-body components

Upstate Medical University

Anatomical Neuroimaging of Caudate nucleus, cerebellum, and ventriclesDouble scoring and entering psychiatric information into databases