Matthew Brenton Email & Phone Number

Aurora, CO

The Charles C. Gates Biomanufacturing Facility (GBF) is a mission-based Good Manufacturing Practices (cGMP) facility. Our mission is to accelerate the translation of scientific discoveries into human clinical trials as safely, efficiently, and cost effectively as possible according to high quality standards.At the GBF, we collaborate with basic science and.

Denver, CO

• Biological method development, routine microbiological testing, and day-to-day operations support for this environmental testing laboratory
• Led laboratory operations to ensure timely sample processing and accurate delivery of client results, within specified turnaround-times
• Led biological method development efforts including molecular analysis, microscopy and classical growth-based assays
• Developed quality management program to better track laboratory inputs and outputs in real time and establish record of compliance with regulatory agencies
• Assisted in client-requested research projects and/or consultation related to quality control
• Generated intellectual property relating to novel methods and processes for analyzing plant material, extracts, and infused-products

Centennial, CO

• Designed and executed client-funded and internal strategic investigations into the complex testing requirements of human cellular and tissue-based therapeutics (HCT/Ps) and biologic drug products. Investigations.
• Implemented Real-Time PCR based Mycoplasma testing in a wide variety of sample matrices, including cell culture based products, and related culture media and sera
• Developed proprietary Real-Time PCR based screening method designed to detect bacterial and fungal pathogens in human tissue recovery swabs with a high degree of sensitivity and specificity in less than 24 hours
• Assisted in culture and characterization of human mesenchymal stem cell (MSC) populations with the end goal of developing functional assays for identity, purity, and potency

Aurora, Colorado

• Used proprietary high-pressure methods to solubilize and refold human proteins for pharmaceutical applications, specifically to reduce protein mis-folding and protein aggregation
• Designed and executed experiments to optimize refolding conditions for different model proteins, using a variety of analytical methods to assess refolding yields including SDS-PAGE and HPLC
• Collaborated with pharmaceutical companies and CMOs to improve their production processes for protein therapeutics; specifically to increase yield, reduce materials costs and time in plant

• Research and Development:
• Conducted high-throughput RNAi screening, for candidate gene discovery. Was instrumental in executing screening projects for clients, from planning phases to confirmation studies
• Delivered microRNA profiling services for clients, and generated final project summaries for profiling results
• Conducted product testing and product research with a focus on quality improvement
• Expanded use of qPCR for gene expression analysis, and developed methodology for offering qPCR confirmation to screening data

• Research and Development:
• Collaborated on internal and external RNAi screening projects, gaining experience with a variety of automated laboratory equipment and cell-based assays
• Performed in vitro experiments using siRNA transfection and lentiviral transduction strategies to modulate gene expression in cell culture systems
• Responsible for method development on automated platforms and troubleshooting to resolve technical difficultiesRNA Production:
• Responsible for custom synthesis, QC analysis, purification, pooling and plating of RNA/DNA samples for client orders. Employed a wide range of scales and modification patterns
• Developed expertise in various robotic liquid handling systems and automated tube/rack management systems, writing new methods and SOPs for high-throughput production

Greater Denver Area

• Studied human genetic variation relevant in lung disease models, with a detailed analysis of newly discovered human gene polymorphism important in the pathophysiology of Acute Lung Injury
• Investigated the genetics of human cognition, by comparing genome-wide insertions and deletions among primate genomes using a variety of hybridization techniques
• Used microarray technology to study the effects of VEGFR-2 inhibitors and Alpha-1-Antitrypsin in rat lungs to model human Emphysema
• Performed genome-wide investigation into LINE-1 retrotransposons in the mouse genome, to build a database of recent LINE-1 insertion events, and to clone and characterize target sites

Iowa City, Iowa Area

• Designed and executed experiments to test surgical spinal implants in the lab, using human cadaver spines and a custom built 3D camera system to measure spinal motion
• Responsible for supervision of student employees and daily laboratory operations
• Responsible for the collection and analysis of data related to investigations into spinal biomechanics, using a variety of spinal instrumentation systems
• Harvested, radiographed and prepared cadaveric spinal specimen for study
• Collaborated in manuscript writing and seeking external funding for projects

Iowa City, Iowa Area

• Separated and analyzed DNA, RNA and proteins to help develop an inhaled antimicrobial treatment for Cystic Fibrosis (using a mouse model)
• Performed various gel electrophoresis techniques including Western, Southern, and Northern blotting
• Performed RT-PCR and various cloning experiments
• Anesthetized laboratory animals and harvested organs for study
• Supported laboratory operations through preparation of reagents, buffers, solvents, etc.

Mba, Bioinnovation And Entrepreneurship

Ms, Biomedical Sciences

Bs, Biological Sciences