Matt Stigliano

Matt Stigliano Email and Phone Number

Research Scientist
Matt Stigliano's Location
Durham, North Carolina, United States, United States
Matt Stigliano's Contact Details

Matt Stigliano personal email

n/a
About Matt Stigliano

Research Scientist with a well-rounded technical background in proteins and neuroscience. I thrive in a collaborative environment, working as a team to produce consistent results and improve the lives of patients.

Matt Stigliano's Current Company Details

Research Scientist
Matt Stigliano Work Experience Details
  • Ucb
    Research Scientist
    Ucb Jan 2022 - Jul 2023
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  • Element Genomics, Inc.
    Research Scientist
    Element Genomics, Inc. Apr 2020 - Jan 2022
    Durham, North Carolina, United States
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  • Ucb
    Research Scientist
    Ucb Dec 2018 - Apr 2020
    Bedford, Massachusetts, United States
    • Purifications of tagged and untagged proteins and antibodies for crystallography and assay from mammalian, bacterial, and insect expression systems• Purifications with RNAse-free and endotoxin-free protocols• Collaboration with project managers to design and execute experiments• Works with lab members to troubleshoot purifications • Coordinates lab safety procedures and hazardous waste disposal
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  • Ucb
    Research Associate Ii
    Ucb Jul 2018 - Dec 2019
    • Conducted and analyzes HPLC of protein complexes• Executed protein analysis with silver staining and western blotting• Performed reagent ordering and biological sample shipments
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  • Beryllium
    Research Associate Ii
    Beryllium Apr 2016 - Jun 2018
    • Learned a variety of purification schemes including affinity, ion exchange, hydrophobic interaction, size exclusion• Utilized microfluidization, sonication, dounce homogenization, Tangential flow filtration, and ultracentrifugation to purify targets• Operated AKTA systems (AKTA FPLC and AKTA Pure) including method design and technical troubleshooting
  • Beryllium
    Research Associate I
    Beryllium Apr 2015 - Apr 2016
    • Performed protein purification using multiple chromatography methods• Worked with project managers to design and execute purification schemes• Maintained equipment critical for production pipeline
  • Umass Medical School
    Graduate Student
    Umass Medical School Jul 2010 - Oct 2013
    Worcester, Ma
    • Performed behavioral assays on an ALS mouse model using the rotarod behavioral assay and testing for weight and survival.• Validated interactions of an ALS-related protein using immunoprecipitation and western blotting.• Screened for isoforms of an ALS gene in N2A cells, HEK cells, and mouse tissue using RT-PCR.• Performed co-transfection experiments in HEK and N2A cells to test co-localization of ALS-related proteins. • Thesis: Characterizing Expression and Function of… Show more • Performed behavioral assays on an ALS mouse model using the rotarod behavioral assay and testing for weight and survival.• Validated interactions of an ALS-related protein using immunoprecipitation and western blotting.• Screened for isoforms of an ALS gene in N2A cells, HEK cells, and mouse tissue using RT-PCR.• Performed co-transfection experiments in HEK and N2A cells to test co-localization of ALS-related proteins. • Thesis: Characterizing Expression and Function of intracellular changes associated with ALS pathology Show less
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  • University Of Massachusetts Amherst
    Researcher
    University Of Massachusetts Amherst Sep 2008 - May 2010
    Amherst, Ma
    • Developed and verified bacterial strains involved in DNA replication• Characterized E. Coli. strains with Immunofluorescence confocal imaging
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  • Morphotek, Inc.
    Process Technician
    Morphotek, Inc. Jun 2009 - Aug 2009
    Exton, Pa
    • Conducted antibody binding experiments for use in breast cancer treatment in pre-clinical trials.• Screened chemicals to increase antibody production in CHO cells and validated using kinetic binding assays (Octet system)

Matt Stigliano Skills

Research Neuroscience Molecular Biology Cell Culture Pcr Microsoft Office Western Blotting Teaching Powerpoint Microsoft Excel Public Speaking Microsoft Word English Windows Photoshop Editing Outlook Protein Purification High Performance Liquid Chromatography

Matt Stigliano Education Details

Frequently Asked Questions about Matt Stigliano

What is Matt Stigliano's role at the current company?

Matt Stigliano's current role is Research Scientist.

What is Matt Stigliano's email address?

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What is Matt Stigliano's direct phone number?

Matt Stigliano's direct phone number is +121589*****

What schools did Matt Stigliano attend?

Matt Stigliano attended University Of Massachusetts Medical School, University Of Massachusetts, Amherst.

What are some of Matt Stigliano's interests?

Matt Stigliano has interest in Science And Technology.

What skills is Matt Stigliano known for?

Matt Stigliano has skills like Research, Neuroscience, Molecular Biology, Cell Culture, Pcr, Microsoft Office, Western Blotting, Teaching, Powerpoint, Microsoft Excel, Public Speaking, Microsoft Word.

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