Maud Contrant Email & Phone Number

Leader of the team emerging viruses and molecular characterization of viral RNAs. - Work on porcine circoviruses and coronaviruses.- Reference laboratory on PEDV research- Research projects focus on i) the role of the RNA secondary structure, cofactors and miRNA on host adaptation ii) recombination process of Nidovirales

Région De Strasbourg, France

Team of Dr. R. MARQUET – J.C. PAILLARTCNRS – UPR 9002 – Virales Ribonucleoproteins, genome incorporation and packagingResearch topicsI am extensively and precisely defining the RNA/RNA interaction network governing the packaging process of the segmented RNA genome of Influenza A virus.Synthetized a novel Reverse Genetics system and optimized of the MIME technic to determine sequences and structures of IAV genome involved in the packaging signals.Skills:• Reverse Genetics: cloning and mutagenesis, synthetic DNA• Viral production, purification and quantification of Influenza A virus • DNA and RNA extraction and preparation• NGS Illumina: libraries preparation and data analysis

Région De Salt Lake City, États-Unis

Team of Dr. S. SWAMINATHANInfectious disease department - EBV and KSHV viruses Research topicsUnderstand the post-transcriptional gene regulation induced by the EBV RNA-binding protein SM protein.The EBV SM protein, which is essential for infectious virus production and has a highly gene-specific mode of action and preferentially enhances expression of several late lytic cycle EBV genes. Developed the iCLIP method to elucidate the interactome of SM protein. Characterize the post-transcriptional regulation of its RNA targets by RT-QPCR and RNAi. Skills:• RNA/protein interactions: iCLIP, immunoprecipitation• qRT-PCR, RNA preparation, RNA pull down, siRNAs• Cell culture (iSLK, AGS cells)• EBV viral infection - purification and titration (flow cytometer)Other skills:Laboratory Safety Training Radiation Safety Training Blood Borne Pathogens Training

Regulation of the expression of the KSHV miRNAsFinalization of some experiments.

Cnrs, Upr9002

PhD (Fellowship from “La Ligue contre le cancer”) Title: Study of the KSHV miRNA biogenesis regulation Team of Dr. S. PFEFFERCNRS – UPR9002 - Non coding RNA and viral InfectionsResearch topicsIdentified the role of RNA secondary structure and action of regulatory proteins into the KSHV miRNA biogenesis regulation.Optimization of the SHAPE and protein identification methods.Skills:• Cell culture: adherent and suspension cells: HEK293T - Bcbl1• Capillary electrophoresis and sequence analysis• RNA Secondary structure determination : SHAPE and probing• Protein identification : immunoprecipitation - affinity chromatography and mass spectrometry analysis• Classical molecular biology and biochemical techniques and enzymology• miRNA expertise

Team of Dr. S. PFEFFERNon coding RNA and viral Infections – UPR 9002 - CNRSResearch topicsDetermination of the expression profil of KSHV miRNAs into different cell lines during viral infection by different methods (High-throughput sequencing- qRT-PCR- quantitative northern blot).

Doctor Of Philosophy (Ph.D.), Molecular Virology And Biology

Master’S Degree, Biology Of Microorganisms: Virology