Maxime Mazille Email & Phone Number

Riehen, Bs, Ch

Suisse

Scientific expert for the molecular diagnostic portfolio. This includes multiple technologies (FISH, arrays and NGS) for hemato-oncology, solid tumors, liquid biopsy and constitutional genetic.NGS panel implementation in diagnostic laboratories:- gDNA and cfDNA isolation.- Nucleic acid quality control.- NGS library preparation for capture-based and amplicon-based UMI workflow.- Operation of Illumina sequencers.- NGS data quality control (fastqc, multiqc).- Somatic and germline variant analysis.- Knowledge of common genetic databases used for variant analysis (gnomad, ClinVar, Ensembl, UCSC, Cosmic...).- Experimental design for troubleshooting and validation.FISH analysis and troubleshooting: - FISH protocol for FFPE, Blood, bone marrow, amniocytes.- Protocol optimization for workflow optimization.- 2-3 colors FISH analysis.Product development: - Collaboration with bioinformaticians for NGS analysis software optimization.Marketing and scientific support activities:- Customer presentation and scientific representation at congresses.- Generation of scientific marketing slide deck for new clinical application in the field of liquid biopsy and for various cancer entities.- Scientific mini-review (Schweizerische Ärztezeitung/Bulletin des médecins suisses) and blog article writing.

Bâle, Suisse

Région De Basel, Suisse

I worked on a project aiming to define the effect of various types of neuronal stimulation on RNA alternative splicing programs, especially on intron retention, a type of alternative splicing only starting to be thoroughly explored.- RNA sequencing experimental design, execution, quality control- Gene expression level analysis- Isoform level analysis- Bioinformatic pipeline optimisation R and UNIX- Statistical analysis of large dataset- PCR, RT-PCR, RT-qPCR- RNA work (sample collection, isolation, purification, RT...)- Western blot - Targeted and shotgun mass spectrometry- Cellular culture (cell lines and mouse primary culture)- Complex vector design, cloning and recombinant protein expression- Drug screening- Immunocytochemistry- fluorescence microscopy and image analysis- Scientific writing and communication- Project management

Westbourne, Angleterre

Région De Bordeaux, France

I worked on a project aiming to develop a model of artificial synapses using micro-patterning via photolithography.- micropatterning-photolithography- mouse primary neuronal culture- Immunocytochemistry- transfection- confocal microscopy- TIRF microscopy- Scientific communication- Scientific writing

Région De Bordeaux, France

I work on a project aiming to define the distribution of various GABAa receptor subunits in the globus pallidus in a rat Huntington disease model.- Confocal microscopy- Immunohistochemistry- Image analysis- FIJI- Scientific communication and writing

Montceau-Les-Mines, Bourgogne, France

- Maintenance of industrial equipment- Ensure constant production- Design and cost evaluation of renovation of the chloration system at the regional level.

Phd, Molecular Biology / Neuroscience, Passed

Esol, English Language B2 Level, Passed

Master'S Degree, Neurosciences And Neuropsychopharmacology, Passed

Bachelor'S Degree, Physiology And Cellular Biology, Passed

Bts, Water Treatment And Technologies, Passed

Degree obtained at the Jacques Duhamel highschool of Dôle (Jura, France) in collaboration with the company VEOLIA EAU