- Enzyme Kinetics- Protein Expression- Cell Culture- Flow cytometry- LC/MS-MS- Fluorescence imagining

• Built and maintained a cell culture library• Isolated stem/progenitor cells from bone marrow• Developed in vitro models of red blood cell development• Utilized flow cytometry to analyze red blood cells/mitochondria• Led and contributed to projects to strengthen the Geriatrics Fellowship Program

• Built and maintained a cell culture library.• Developed expressible and non-expressible excitation ratiometric biosensors.• Developed a system for imaging intra-cellular and intra-mitochondrial zinc during oxygen-glucose deprivation.• Isolated and determined the expression location of a fluorescent protein in mitochondria of transfected cells.• Developed calibration standards for lifetime-based fiber optic fluorescence sensors.• Developed and maintained a laser system for measuring fluorescence intensities and pM to μM changes in Ca2+, Zn2+, and Cu2+.• Measured and calculated fluorescence lifetimes, phase delays, and modulation ratios of various samples through fiber optic cable up to 1km in length.

• Synthesized and purified new compounds to be tested for anti-neoplastic and hypolipidemic activity in vivo and in vitro.• Maintained a small animal testing facility.• Executed solid phase parallel synthesis of target compounds.• Executed fluorous phase parallel synthesis of target compounds.• Characterized new compounds via 13C NMR, 1H NMR, IR, UV, and physical properties