Cell engineering, stable cell line generation, CRISPR, process development, high throughput screening, automation development, AAV

- Thesis title: Engineering photosynthetic bacteria as factories for the sustainable manufacturing of vitamins and medications- Developed a novel axenicity method for Limnospira fusiformis (previously named Arthrospira platensis)- Sequenced L. fusiformis using Oxford Nanopore and Illumina technologies- Developed an L. fusiformis DNA extraction method- Integrated genes for vitamin B12 and acetaminophen synthesis into the Synechococcus elongatus genome - Extracted and purified vitamin B12 from cyanobacteria cells- Detected vitamin B12 and acetaminophen via HPLC and microbial methods- Key methods: Cyanobacteria culturing, homologous recombination, genomic integration, genetic engineering, DNA extraction, molecular cloning, PCR, next generation sequencing (NGS), HPLC

- Aimed to improve the nutritional value of Limnospira fusiformis (Spirulina) by completing the cobalamin (vitamin B12) synthesis pathway. - Designed genetic edits to induce production of cobalamin in Synechococcus elongatus, a model cyanobacteria- Established cobalamin detection and quantification methods using a riboswitch and HPLC- Set up the lab for cyanobacteria culturing - Corresponded with international healthcare professionals- Assisted in community outreach and educational programs- Worked collaboratively with a team of 15 undergraduates

Assisted in immunogenomic research and B cell sequencing to study the adaptive immune system via RT-PCR, sequencing, and Python.

Organized and maintained law libraries, documents, and case files. Organized and prioritized the case loads of two attorneys.