Md. Ehtashamul Haque Email & Phone Number

Dhaka, Bd

Bangladesh

Major Works:•Implemented technical knowledge during new facility design & HVAC layout design •Optimized and scaled up upstream production process of rh-Insulin (using cloned Pichia Pastoris), rh-Interferonα2a, rh-GCSF and recombinant Ranibizumab (using cloned E. Coli BL21).•Maintained the master cell bank and prepared working call bank (cloned E. Coli and Pichia pastoris) for the upstream production of recombinant proteins. •Maintained the mammalian cell bank (e.g. NFS-60, MDBK, Raji, HUVEC etc.) for Bioassay. •Quality Control and In-Process quality analysis of the above proteins (during process development).•Writing & reviewing process validation protocols, BMR’s, SOP’s, generating result sheets and preparing other documents related to QC.•Supervising the cleaning and environment monitoring of ‘B’ and ‘C’ grade clean area.•Maintaining the inventory of R&D.Specialties acquired on process and analytical equipments:•Fermentor 100L & 450L (Bioengineering), 14L (New Branswick BioFlow310); Bioreactor Sartorius STR200.•Cell Disruptor (Constant Systems, UK and GEA Niro Soavi, Italy)•TFF System (Flex-Stand system, GE healthcare and Cassette system,Pall Life Sciences)•Batch Centrifuge – 6L & Continuous Centrifuge (GEA Westfalia)•AKTA pilot (GE healthcare); AKTA ready (GE healthcare); HPLC (Shimadzu & Dionex)•Real Time PCR (Applied Biosystems,USA); Gel Doc system •Spectrophotometer, ELISA reader & Fluorescence reader Specialties acquired on laboratory analysis:•Bradford, Lowry and BCA protein quantification; Bacterial endotoxin test (LAL test) •SDS PAGE; Isoelectric focusing; Western blot; Slot blot; ELISA•DNA detection by Picogreen method and Real-Time PCR method•Biological assay (Interferon, Filgrastim, Rituximab, Adalimumab, Trastuzumab, Darbepoetin, erythropoietin)•HPLC (peptide mapping, related protein, size exclusion chromatography)

• Daily analysis of water samples as: Membrane filtration (for E. coli & total coliforms) H2S testing (for fecal contamination) Free Chlorine testing (using digital colorimeter)• QA/QC procedures for all water quality analysis performed.• Input water quality results into digital format and assist in cleaning up and interpreting data.• Daily coordination with field staff to receive water samples collected from study participants. • Maintenance of field laboratory facility in fully functioning order at all times

• Sample handling: Sample processing, Preservation and coordination with the field staffs• Microscopic observation of different intestinal parasites• Cell culture: Different microbial cell culture as well as human lymphocyte cell culture• Immunological tests: ELISA, Immunofluorescence Assay etc.• Molecular techniques: DNA & RNA extraction by different methods, Conventional PCR, Real-time PCR (BioRad).• Data management: Maintaining log book, maintaining data found from different experiments, analysis of data and interpretation of results

Master Of Science (M.S.), Biochemistry And Molecular Biology, 1St Class (61% Marks)

Bachelor Of Science (B.Sc.), Biochemistry And Molecular Biology

Higher Secondary Certificate, 5.0 Out Of 5.0

Secondary School Certificate, Science, 4.38 Out Of 5.0