Since 2007, my lab has been involved in sequencing a number of bacterial, fungal and plant genomes. With surprise, we found that our experience on sequencing assembling and annotating the genome of "difficult" fungi and plants allowed us to smoothly approach human whole genome sequencing (WGS) and interpretation. Thanks to a lab composed by fantastic and enthusiastic people with different skills and very much different backgrounds (bioengineers, human and plant bioinformaticians, computer scientists, biotechnologists and geneticists), we are now taking full advantage of our understanding of the complexity of different living organisms. It's amazing to see how much it can be learned when in the same lab people work on bacteria, plants and humans. Comparison of the different technologies available and testing of new emerging technologies for DNA sequencing and analysis is an integral part of our mission. Whereas the lab is focused on genomic and transcriptomic projects with a special focus on the characterization of the "private" genome that we believe strongly contributes to make the difference among living organisms belonging to the same specie, I'm particulary interested in human genome interpretation. I sequenced myself in 2011, and in 2013 I've joined the Understand Your Genome (UYG) program, a movement promoted by Illumina towards a better understanding of our DNA with the mission of accelerating a true era of precision medicine. I've then served UYG as Key Opinion Leader (KOL) to help catalyzing a cross-professional community united to responsibly yet urgently accelerate the adoption of genomics in healthcare.Another field in which I am particularly active is related to on site species identification for biodiversity studies and wildlife conservation. We keep developing a mobile DNA sequencing laboratory that was proven successful for sequencing in extreme field conditions, such as in the tropical rainforests of Tanzania, Congo and Borneo.