Adam Mefferd Email & Phone Number

Durham, North Carolina, United States

Durham, North Carolina, United States

· Designed and managed multiple independent research projects simultaneously and maintained accurate experimental records in the ELN· Supervised the production of AAV by two junior research associates· Studied how the AAV D-sequence contributes to genome polarity helping to drive key program decisions· Optimized triple plasmid transfection protocol resulting in >10-fold increase in AAV production· Developed engineered cell lines using CRISPR resulting in ~30% improvement in AAV yield· Collaborated with the NGS team to develop a platform for the sequencing of full-length AAV genomes using nanopore technology· Developed an optimized ultracentrifugation process for the purification of self-complementary AAV· Regularly presented findings at both group and company-wide meetings · Authored formal technical reports regarding improvements to AAV production and characterization processes

Durham, North Carolina, United States

· Produced research grade AAV for in vivo studies from up to 5L of suspension HEK293 culture while maintaining strict deadlines for AAV vector production and delivery· Implemented an AKTA chromatography-based AAV purification process substantially improving production throughput and efficiency · Identified and removed sources of endotoxin contamination within the AAV purification process· Trained junior employees in upstream and downstream production of AAV· Investigated the immunogenicity of the AAV genome by analyzing CpG depleted AAV vectors in rats· Worked closely with vendors to acquire reagents and products on strict timelines.

Durham, Nc

· Produced research grade AAV for use in vitro · Maintained critical reagents and equipment necessary for AAV production· Wrote and updated SOPs and maintained detailed records of AAV productions· Investigated the viability of a self-inactivating AAV for use in vivo

· Designed and managed multiple independent research projects simultaneously · Maintained accurate experimental records and laboratory SOPs · Developed novel CRISPR sgRNA expression technology leading to a successful patent.· Performed a broad range of molecular biology techniques including qPCR, SDS-PAGE, western blotting, northern blotting, cloning, and next generation sequencing· Performed experiments with custom AAV and lentiviral vector systems in order to deliver gene editing components to cellular targets· Developed expertise in CRISPR/Cas based gene editing technology using numerous Cas9 enzymes and single-guide RNA combinations· Mentored both undergraduate and graduate students in laboratory procedures and techniques

Columbus, Ohio Area

· Performed basic laboratory maintenance procedures and cell culture techniques. · Performed qRT-PCR on mRNA harvested in vivo to determine mouse cytokine levels after latent CMV infection· Collaborated with physicians in the Department of Surgery to investigate pathology of CMV infection

Bachelor Of Science - Bs, Microbiology, General

Bachelor Of Arts - Ba, Chemistry