Megan Settle Email & Phone Number

Cleveland, Oh, Us

• Direct the building and design of the R&D and microbiology laboratory from the ground up. • Design laboratories to meet all biosafety, personal protective requirements and to maintain the stability of raw components and finished products.• Oversee the selection of equipment and materials • Develop and maintain work flow process to streamline batch production from raw materials to finished products to microbiological testing. • Create FDA-compliant standard operating procedures from scratch• Challenge test products to determine viability of the preservatives used.• Develop environmental challenge testing to determine product stability.• Adhered to Good laboratory practice (GLP) techniques to ensure rigor, consistency, and safety during manufacturing and testing • Laboratory Techniques Gained & Utilized: o Designing assays to identify pertinent bacteria, yeasts and moldso Breaking down oil based, powder based and water based products for testingo Analyzing and choosing preservatives o Deconstructing and reconstructing cosmetic formulations (oil, powder and water based)o Accurate testing and record keeping according to FDA standardso Dissecting formulations according to chemical composition to optimize productiono Maintaining laboratory equipmento Providing support and mentoring in the R&D laboratory

Cleveland/Akron, Ohio Area

• Determine and execute methods for improving the safety, efficacy, tolerability, and overall quality of the compounded medications currently produced.• Develop compliance guidelines and standard operating procedures according to Pharmacy State Board regulations, FDA regulations and the U.S. Pharmacopeia.• Underwent FDA, California, and Ohio inspections, both for opening the pharmacy in a new location and annual inspections.• Collaborative research with outside institutions. Ensuring all goals were accomplished according to grant proposals in correlation with our set compliance standards.• Research, suggest and implement equipment and methods that improve efficiency in the preparation and packaging of medications; including capsules, emulsions/lotions and syrups.• Work closely with outside testing laboratories to ensure that the potency and stability of the medications tested is performed correctly.• Work closely with pharmacists in real time to ensure compounded medicine is compliant with changing regulations.• Continuously audit the compounding processes to determine areas of inefficiency and suggest improvements. • Compliance monitor following USP guidelines.• Act as secondary compliance officer.

Cleveland/Akron, Ohio Area

• Human Heart transplant processing• Performed and analyzed RT-PCR on Affymetrix platform• Conducted biofeedback analysis• Laboratory Techniques Gained & Utilized: ∙ Transporting and dissecting whole hearts and left ventricular device cores for experimentation and storage.∙ Removed trabecular muscles for experimentation∙ Performed isometric contractile function tests to determine post-rest potentiation and index of inotropic response to beta-adrenergic stimulation and muscarinic agonists.∙RNA isolation ∙Provided pre- and post- biofeedback-assisted psychophysiological assessments to participants as part of a research study examining the additive benefits of biofeedback-assisted physiological self-regulation in stress management training. Assessments included measurement of respiration, skin temperature, electrodermal activity, electromyography, and heart rate variability.

Cleveland/Akron, Ohio Area

Teaching undergraduate students molecular biology. Writing and implementing lectures and exams. Topics covered•Mitosis •Protein translation•Meiosis •Regulation of gene expression•Chromosomal inheritance •Viruses •Molecular inheritance •Genome evolution

I performed various experiments in relation to molecular cardiology. The main focus of my laboratory research was studying kindlins 2 and 3 in relation to integrins. Working in a multicultural environment allowed me to interact with great scientists from all over the world. I was responsible for the following within the laboratory. • Manage mouse colony of over 60 specific backgrounds •Handle adverse events within the colony regarding genomic problems, health issues and breeding issues•Work with multiple doctors as support for transgenic design, experiment design and implementation•Monitor and report the experimental progress•Maintain colony records and report to Internal Review Board•Trained in handling adverse events within governing entity•Maintain extensive database correlating lineage of colony• Laboratory Techniques Gained & Utilized:∙Non-invasive heart puncture ∙Tail vein injection∙CBC analysis ∙Neurological behavioral tests∙Blood smear techniques ∙Reticulocyte staining∙Wound study design and implementation ∙BSL2 facility trained

Coordinated execution of assigned protocols in accordance with research governing entity •Collaborated with primary investigator, and research staff to plan, conduct and evaluate project protocols •Monitor and report on progress of projects•Assisted with recruitment, selection and monitoring of research project participants•Trained in handling adverse events and compliance issues•Assists with responding to and preparing for audits• Interacted with study participants on a daily basis• Collaborated with statisticians and data base designers to implement study changes

Pathology And Laboratory Medicine Institute

Skilled in Epic, Copath, and Navicare patient information systems•Skilled in data analysis utilizing GraphPad Prism and Excel software•Developed and implemented novel procedures for laboratory•Skilled in writing standard operating procedures for laboratory•Trained residents and fellows in laboratory procedures•Trained in automated immunohistochemistry staining and manual staining•Developing and maintaining extensive research sample databases• Laboratory Techniques Gained & Utilized:∙Processing human blood samples to obtain serum, plasma, and buffy coat ∙Human Tissue DNA extraction ∙Fresh tissue epithelial cell isolation∙PCR DNA quantitation ∙Colon, Liver and Stomach tissue procurement and banking∙ laboratory equipment repair and upkeep ∙FISH (Fluorescent In Situ Hybridization) ∙Paraffin tissue cell isolation∙FISH Touch slide preparation ∙FISH nuclear slide preparation ∙FISH manual microscopic enumeration ∙Frozen tissue cryostat sectioning ∙Growing and fluorescently labeling bacterial artificial chromosomes ∙Paraffin tissue sectioning

Trained incoming employees and students• Radioactive training • Trained in the left arterial descending ligation surgery in mice • Specifically trained in the removal and isolation of aortic endothelial and smooth muscle cells • Laboratory Techniques Gained & Utilized: ∙Lipid metabolism ∙Cellular lipid efflux • ELISA∙Primary mouse macrophage cultures ∙Immunohistochemistry∙Fluorescent microscopy ∙PCR cycle sequencing∙Fluorescent and chemiluminescence imaging ∙Western blotting∙Agarose gel electrophoresis ∙Sterile techniques for cell culture∙proficient in making chemical solutions ∙Breeding and weaning of research mice∙Mouse surgery for Quantitation Trait Locus study ∙Transient transfection,∙Mini and maxie plasmid preparation generation of stable transfected cell lines and quantification of plasmids

Provided research support in molecular studies to determine the effects of Type I and Type II diabetes in transgenic mice• Laboratory Techniques Gained & Utilized:∙DNA quantification ∙Labeled DNA probes ∙Non- radioactive DNA sequencing ∙cDNA library screening∙Non-radioactive signal detection ∙Made DIG probes by PCR∙Nucleic acid hybridization assays ∙Immunology techniques∙Northern and Southern Blotting ∙Nucleotide data base searching∙Breeding and weaning of transgenic mice

Assisted thirty-five residents in a floor section• Coordinated educational programs, promoted a community, and administered university policies

Masters Of Biology, Molecular Biology

Advancements in Molecular Biology Biochemistry Biomedical Physiology Chromosome Structure and Function - Graduate Seminar; Science.

Bachelor Of Science, Microbiology

Molecular Genetics - Quantitative Analysis - Laboratory Genetics - Pathogenic Bacteriology - Microbial Physiology - Immunology - Microbial.