- I contribute to development projects through hands-on independent work of highly complex tasks involving multiple experimental activities and groups; Analyze complex data with high degree of sophistication and set direction on experiments, independently prepare presentations and SOPs.- I collaborate with cross-functional project teams, reporting and discussing research results to make recommendations and support strategy shaping; Mentoring junior colleagues and research associates.- Product development experience: · Cell line engineering and sorting including human induced pluripotent cells (hiPSC). · Product verification and validation test (V&V). · Reagent sustained quality control (QC). · Reagent stoichiometry. · Point-of-contact person for cross-functional team collaboration.- Assay development experience (Delivered multiple assays and SOPs): · Instant cell based DNA/RNA quantification workflow. · Gene editing detection and target cell sorting. · Ribonulceoprotein complex activity detection assay. · Innovative NGS library preparation workflow with crude cell lysate.

• Trailblazing projects by developing and employing state-of-the-art methods include enhancer RNA detection, functional genomics, Chromatin Conformation Capture (3C), CRISPR-editing, total RNA sequencing, and Chromatin Immunoprecipitation followed by Sequencing (ChIP-Seq) to comprehensively elucidate the epigenetic program of enhancer RNAs upon hormone stimulation in mammalian breast cancer cell models.• Proficient communicator with ability to effectively deliver accurate scientific concepts and results to diverse audiences, proven by 3 oral talks and 3 poster presentations at international conferences and department meetings. Wrote and published first-author and co-author papers in high impact peer reviewed professional journals.- Project experience: · Functionality study of enhancer RNAs. · Functional genomic for breast cancer drug resistant. · Prostate cancer drug resistant through transcription factor rearrangement.- Assay innovation and development experience: · ChIP-seq, RNA-seq, GRO-seq. · Next Generation Sequencing (NGS): library construction and QC, workflows innovation and optimization. · CRISPR editing: gRNA design, cell transduce, validation by NGS. · High throughput Pooled CRISPRi screening, with Lentivirus library. · In vitro transcription; Recombinant DNA technology. · Protein expression, purification, and immunoprecipitation. - Writing & Communications: · First-author and co-author papers in high impact peer reviewed journals. · Collaborate with clinician, bioinformatician and wet lab scientists. · Talks and posters presentations at international conferences. · Mentor Ph.D. candidate students and research associates.

•Highly independent researcher who conceives and proposes projects, applies for funding, collects and analyzes data, leads the research group, and successfully completes the projects, evidenced by being issued the PCRP Early Investigator Research Award from DOD for the Fiscal year of 2016 as one of only sixteen awardees nationwide as well as a proven track record of publications.•Design all the experiments according to the project workflow from the macro perspective while executing the assays with extremely meticulousness to elucidate the chromatin structure remodeling function of bromodomain-containing protein. Address and submit period reports and the final report under the regulation of DOD.

I built predictive biomarkers from various sources of clinical samples, provide strategies for favoring treatments of cancer or early detection of cancer.- Project experience: · Identification of gastric cancer specific biomarkers from tissue samples. · Cell-free miRNA signature for early diagnosis of colorectal cancer.- Assay development experience: · Immunohistochemistry (IHC) staining: biopsy chips. · Cell-free miRNA extraction and quantification; Real-time qPCR. · Work with clinical samples.

•Comprehensively employ the technique of “omics”, proteomics (Two-Dimensional Electrophoresis followed by MODI-TOF/TOF) and genomics (DNA microarray) to identify, analyze and organize the signaling crosstalk in human gastric cancer cell lines upon manipulating the status of the inflammatory protein nuclear apoptosis inducing factor1 (NAIF1), •Making use of Confocal microscopy, Fluorescence Activated Cell Sorting (FACS), immunoblot, as well as establishing mouse xenograft models to assess the in vitro and in vivo anti-tumor effect of NAIF1.